1. BIOSENSOR

2. A biosensor is a device for the detection of an analyte that combines a biological component with a physicochemical detector component. It consists of 3 parts. 1. Biological material (eg. tissue, microorganisms, organelles, cell receptors,enzymes, antibodies, nucleic acids, etc), a biologically derived material or biomimic. The sensitive elements can be created by biological engineering.

3. 2. Transducer or the detector element (works in a physicochemical way; optical, piezoelectric, electrochemical, etc.).
It transforms the signal resulting from the interaction of the analyte with the biological element into another signal (i.e., transducers) that can be more easily measured and quantified.
3. Associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way.

4. A common example of a biosensor is the blood glucose biosensor.
It uses the enzyme glucose oxidase to break blood glucose down.
It first oxidizes glucose and uses two electrons to reduce the FAD (a component of the enzyme) to FADH2.
This in turn is oxidized by the electrode (accepting two electrons from the electrode) in a number of steps.
The resulting current is a measure of the concentration of glucose.

5. In this case, the electrode is the transducer and the enzyme is the biologically active component.
Many of today's biosensor applications are similar, in that they use organisms which respond to toxic substances at a much lower level than us to warn us of their presence.
Such devices can be used in environmental monitoring, trace gas detection and in water treatment facilities.

6. Applications
Glucose monitoring in diabetes patients
Environmental applications
e.g. the detection of pesticides and river water contaminants
Detection of pathogens
Determining levels of toxic substances before and after bioremediation
Detection and determining of organophosphate

7. Routine analytical measurement of folic acid,biotin, vitamin B12 and pantothenic acid as an alternative to microbiological assay
Determination of drug residues in food, such as antibiotics and growth promoters, particularly meat and honey.
Drug discovery and evaluation of biological activity of new compounds.
Detection of toxic metabolites such as mycotoxins.

8. Biosensors in food analysis
In food industry optic coated with antibodies are commonly used to detect pathogens and food toxins.
The light system in these biosensors has been fluorescence, since this type of optical measurement can greatly amplify the signal.

9. A range of immuno- and ligand-binding assays for the detection and measurement of small molecules such as water soluble vitamins and chemical contaminants (drug residues) such as sulfonamides and Beta-agonists have been developed, often adapted from existing ELISA or other immunological assay.
These are in widespread use across the food industry.

10. Biosensors in fisheries
Problems of poor water quality affect most developed countries in the world, the contaminants stemming largely from industrial waste and sewage.
The effects of these pollutants have already been noted, particularly in fish, which often show reproductive dysfunction with males displaying feminization.
The biochemical responses of organisms to organic and metal compounds in the water can be measured and used as a biomarker for the level of pollution.

11. Most commonly, Cytochrome P4501A is used as it is responsive to a number of organic chemicals including aromatic hydrocarbons and dioxins.
The induction of this gene by these contaminants measured by changes in protein expression or mRNA levels.
Alternatively, metallothioneins are utilized, which are induced specifically by metals.

12. Similarly, gene encoding green fluorescent protein (GFP) is fused to a number of promoters which will respond to water pollutants.
These include the promoters from some inducible genes such as: those encoding heat shock proteins or metallothioneins which are induced by general stress, heavy metals or chemical toxins, those contain estrogen response elements being induced by estrogens or xenoestrogens.

13. The availability of the GFP as a reporter gene has enabled the use of transgenic organisms as biosensors for water contamination providing rapid and visible results while eliminating the need for enzymatic or specific protein assays.

14. Thank you