1. General imprinting status is stable in assisted reproduction–conceived offspring 
Chun Feng, M.D., Shen Tian, Ph.D., Yu Zhang, M.D., Jing He, M.D., Xiao-Ming Zhu, Ph.D., Dan Zhang, Ph.D., Jian-Zhong Sheng, Ph.D., He-Feng Huang, M.D. 
Fertility and Sterility 
Volume 96, Issue 6, Pages e9 (December 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Expression profile of imprinted genes in umbilical cord blood specimens of ART- and naturally conceived offspring by microarray and real-time RT-PCR. (A) Unsupervised hierarchic clustering of imprinted genes. Rows represent genes, and columns represent samples. Colored pixels capture the magnitude of the response for any gene, where red, green, and black represent induction, repression, and no change, respectively. There was no obvious clustering of the two groups. N = naturally conceived offspring; IVF = IVF-conceived offspring; ICSI = ICSI-conceived offspring. (B) Real-time RT-PCR for imprinted genes differentially expressed. Each column and bar represents the mean and standard error, respectively. *P<.05. (C) Microarray and real-time RT-PCR for the three differentially expressed imprinted genes. The y-axis and x-axis represent the RT-PCR and the microarray results, respectively. For the real-time RT-PCR results, only the data of 16 samples involved in the microarray assay (four IVF-, four ICSI-, and eight naturally conceived children) are shown.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Imprinting of PEG10, L3MBTL, and PHLDA2 genes of ART- and naturally conceived offspring. The left and right columns represent the sequencing result of gDNA and cDNA, respectively. (A, C, E) Results of children conceived naturally. (B, D, F) Results of children conceived through ART.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Methylation profile of L3MBTL differentially methylated region. Methylated and unmethylated CpGs are shown as solid and open circles, respectively. (A) All of the CpGs were moderately methylated in one naturally conceived child. (C) The 15th CpG showed hypomethylation in one IVF case. (E) All of the CpGs were completely unmethylated in one ICSI case. (B, D, F) Summary of bisulfite-sequencing PCR (BSP) results of naturally, IVF-, and ICSI-conceived children. (G) BSP results of the parents of the child with LOI.
Fertility and Sterility  , e9DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions