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Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.

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Presentation on theme: "Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth."— Presentation transcript:

1 Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth Durso  The Journal of Molecular Diagnostics  Volume 7, Issue 5, Pages (November 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Fluorescence measurements derived using the SmartCycler for 10-fold serial dilutions of GBS (5 to 50,000 CFU/ml) spiked into neonatal whole-blood samples and analyzed by 16S rDNA PCR. Site ID numbers represent samples tested in duplicate at the following concentrations: A1 to 2, 5 × 104 CFU/ml; A3 to 4, 5 × 103 CFU/ml; A5 to 6, 5 × 102 CFU/ml; A7 to 8, 50 CFU/ml; A9 to 10, 5 CFU/ml; and A11 to 12, negative controls lacking target DNA. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 A plot comparing the ΔCT values of the Cx+/PCR+ specimens, in ascending order, with those of the Cx−/PCR− specimens, in descending order. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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