1. Anaesthetic preconditioning but not postconditioning prevents early activation of the deleterious cardiac remodelling programme: evidence of opposing genomic responses in cardioprotection by pre- and postconditioning#  
E. Lucchinetti, R. da Silva, T. Pasch, M.C. Schaub, M. Zaugg 
British Journal of Anaesthesia 
Volume 95, Issue 2, Pages (August 2005)
DOI: /bja/aei155
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

2. Fig 1
Scheme of treatment protocols. A_PreC: anaesthetic preconditioning with isoflurane (2.1 vol% for 15 min followed by 10 min of washout) prior to 40 min of test ischaemia. A_PostC: anaesthetic postconditioning with isoflurane (2.1 vol% during the first 15 min of reperfusion) after 40 min of test ischaemia. Ischaemia: non-preconditioned hearts exposed to 40 min of ischaemia. Control: time-matched perfusion. In each group, five hearts were used for microarray analysis. Additional five hearts per group were used for infarct size measurements.
British Journal of Anaesthesia  , DOI: ( /bja/aei155)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

3. Fig 2
Infarct size measurements. Hearts were sliced into 2 mm cross-sections and stained with triphenyltetrazolium chloride 1%. *P<0.05 compared with unprotected hearts (ISCH). A_PreC:= anaesthetic preconditioning. A_PostC:= anaesthetic postconditioning.
British Journal of Anaesthesia  , DOI: ( /bja/aei155)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

4. Fig 3
The Venn diagrams show the numbers of differentially up- and downregulated transcripts, as obtained by significance analysis of microarrays (SAM), and the number of overlapping transcripts between groups.
British Journal of Anaesthesia  , DOI: ( /bja/aei155)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

5. Fig 4
Coupled two-way cluster (CTWC) analysis. (a) Global gene expression matrix (heat map) of the 2133 anova-filtered genes. Rows correspond to the genes, and columns correspond to the samples. The color scale denotes the quantification for gene expression based on normalized and centered log2 RMA values. Blue indicates least and red greatest degree of expression. CTWC provides dendrograms of gene and sample clusters. Each circle of the dendrogram represents a cluster. The gene dendrogram of the eight stable gene clusters is depicted on the left of the matrix. Note that clusters G3 and G4 are subclusters of cluster G5, and G2 is a subcluster of G7, showing common parents in the tree. The sample dendrogram with the two stable sample clusters is shown at the bottom of the matrix. Major splits marked with circles indicate stable clusters. Red digits indicate stability of gene and sample clusters as derived from the algorithm. (b) Distance matrix reordered according to genes. Blue spots on the diagonal axis indicate gene clusters. (c) Principal component analysis of the various treatment groups. Note that A_PreC clusters with healthy virgin myocardium.
British Journal of Anaesthesia  , DOI: ( /bja/aei155)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

6. Fig 5
Expression patterns of the six most relevant stable gene clusters resulting from coupled two-way clustering. Clusters G2, G3 and G4 are subclusters. Groups are indicated on the x-axis. The number of genes of the individual clusters and corresponding stability index ΔT, over which the clusters remain stable, are given on the y-axis. The corresponding gene lists can be found in the supplementary material (file CTWC_results.xls).
British Journal of Anaesthesia  , DOI: ( /bja/aei155)
Copyright © 2005 British Journal of Anaesthesia Terms and Conditions