1. TAMUCC Biology & Microbiology Lab Safety Orientation Science & Engineering

2. BIOLOGY & MICROBIOLOGY LAB SAFETY ORIENTATION
Overview
Emergency Phone Number
Emergency Evacuation
Accident Reporting
Lab Attire
Hazard Communication: Chemicals & Biohazards & Glassware
Personal Protective Equipment
First Aid

3. LAB SAFETY ORIENTATION
Campus Emergency: Call University Police Department (UPD) in case of an emergency.
From any university phone: dial 911 or ext
From your cell phone: dial

4. LAB SAFETY ORIENTATION
Emergency Call:
If you first call 911 using your cell phone, make sure to also notify UPD at (361) so they can assist in the response.

5. LAB SAFETY ORIENTATION
At the beginning of the semester:
Become familiar with your building;
Know your fire exits;
Know the shortest route to the nearest exit;
Be aware of your surroundings;
And…

6. LAB SAFETY ORIENTATION
Fire Evacuation Procedures - in case of a fire:
Exit the building in a calm & orderly manner.
Use the shortest and safest passage to the exit.
DO NOT RUN.
Do not use the elevator.

7. LAB SAFETY ORIENTATION
Fire Evacuation Procedures
Alert people and pull the fire alarm on your way out.
Meet your class at the assembly area designated by your Instructor.
Stay at least 100 feet away from the building (or 35 long steps)

8. LAB SAFETY ORIENTATION
Fire Evacuation Procedures
Do not re-enter the building until the “All Clear” has been announced.
Regardless of whether it is a real fire alarm, false alarm, or a fire drill, you are required to follow the same procedures above.

9. LAB SAFETY ORIENTATION
Fire Evacuation - Helping a person with mobility impairment:
Accompany him/her to an area of rescue assistance located at the stairwell landing.
Call or have someone call UPD & give UPD the location.

10. LAB SAFETY ORIENTATION
Helping a person with mobility impairment:
UPD will bring him/her down the stairway using an evacutrak.
Stay with him/her until UPD arrives unless your life may be in danger.
Do not use the elevator.

11. LAB SAFETY ORIENTATION
Fire Safety
Never leave a fire unattended.
Be aware of Bunsen burners emitting invisible flames.
Keep flammable & combustible materials away from a heat source.

12. LAB SAFETY ORIENTATION
Fire Safety
Report all malfunctioned equipment to your Instructor.
Do not use frayed, damaged electrical cords.
Do not overload the circuit with an “electrical octopus.”

13. LAB SAFETY ORIENTATION
Accident Reporting – Life Threatening
For a life-threatening incident, contact UPD
From any university phone, dial 911 or 4444.
If an ambulance is needed, inform UPD so.

14. LAB SAFETY ORIENTATION
Emergency Call
If you first call 911 using your cell phone, make sure to also notify UPD at so they will assist in the response.

15. LAB SAFETY ORIENTATION
Accident Reporting – Non Life-Threatening
Report all non life-threatening incidents to your Instructor.
Use the first aid kit in the lab.
Go to the Health Center if needed.

16. LAB SAFETY ORIENTATION
Lab Attire: Upon entering the lab, you are required to wear:
Lab coat
Long pants
Sturdy closed-toe closed-heel shoes

17. LAB SAFETY ORIENTATION
Lab Attire
Shoes should cover your feet as much as possible.
Leather top & slip-resistant sole are also recommended.
Without proper attire, you will be asked to leave.

18. LAB SAFETY ORIENTATION
Lab Safety Behavior
NO FOOD, NO GUM
NO DRINK
NO COSMETICS
Never touch, taste, or smell any chemicals
Long hair tied back
No horseplay (pushing, shoving, pranks, etc.)

19. LAB SAFETY ORIENTATION
Emergency Equipment:
Fire extinguisher or other emergency equipment may be available inside the lab.
However, using them is only on a voluntary basis.

20. LAB SAFETY ORIENTATION
Emergency Equipment: Use emergency equipment only if:
You are trained to use them.
Your own safety is not compromised by using them.
Remember: YOUR OWN SAFETY COMES FIRST.

21. LAB SAFETY ORIENTATION
Emergency Phone in your lab.
When the red light is flashing and/or the phone is ringing, there may be an emergency announcement.
PLEASE PICK IT UP IMMEDIATELY!

22. LAB SAFETY ORIENTATION
Working With Chemicals
Getting to know your chemical before using it.
There is a Material Data Safety Sheet for every chemical in your lab.
Looking at a MSDS is the best way to get to know your chemical.

23. LAB SAFETY ORIENTATION
The Material Safety Data Sheet (MSDS) provides information regarding the chemical’s
Physical, chemical, health properties
Safe handling, personal protective equipment required, disposal, storage, etc.

24. LAB SAFETY ORIENTATION
NFPA Fire Diamond is another way to communicate chemical hazards
NFPA
Diamond

25. LAB SAFETY ORIENTATION
Potential Health Hazards of Chemicals
Poisons: Chemicals capable of harming a person in a small amount.
Asphyxiants: Chemicals capable of depriving the body of oxygen.
Sensitizers: Chemicals capable of causing an allergic reaction upon repeated exposures

26. LAB SAFETY ORIENTATION
Potential Health Hazards of Chemicals
Carcinogens: Chemicals capable of producing/causing cancer in humans or animals.
Reproductive Toxins: Chemicals capable of affecting the male or female reproductive system or a developing fetus.

27. LAB SAFETY ORIENTATION
Potential Health Hazards of Chemicals
Irritants: Chemicals capable of causing inflammation, redness or itching upon exposure.
Corrosives: Chemicals capable of destroying or causing irreversible damage to the skin, respiratory system, eyes.

28. LAB SAFETY ORIENTATION
Potential Physical Hazards of Chemicals
Cryogens: Extremely cold chemicals capable of causing fire or explosion.
Flammables: Chemicals capable of causing fire or explosion.
Oxidizers: Chemicals capable of providing oxygen to feed a fire or explosion.

29. LAB SAFETY ORIENTATION
Routes of Entry: Paths that chemicals can get into your body.
Inhalation Route: Contaminants enter the body through the lungs.
Absorption Route: Contaminants enter the body through the skin or eyes

30. LAB SAFETY ORIENTATION
Routes of Entry:
Ingestion Route: Contaminants enter the body through the digestive system.
Injection Route: Contaminants enter the body through a contaminated sharp object that breaks through skin.

31. LAB SAFETY ORIENTATION
Hazard Communication
Learn about the chemicals before using them
DO NOT use any chemicals without your Instructor’s supervision or approval.
Do wear all personal protective equipment as required.
When in doubt, ASK.
propylene

32. LAB SAFETY ORIENTATION
Personal Protective Equipment (PPE)
You will be required to wear PPE when working with certain chemicals.
Follow your Instructor’s instruction.

33. LAB SAFETY ORIENTATION
Eye Protection:
Goggles are required while you work with chemical in the lab.
Put them on properly and keep them on while working with chemical. Goggles on your forehead WILL NOT protect your eyes.

34. LAB SAFETY ORIENTATION
Hand Protection:
Gloves are required while you work with chemical or biological agents in the lab.
Use the gloves provided to you in the lab. They are compatible with agents you work with.

35. LAB SAFETY ORIENTATION
Chemical in Eye:
Flush eyes out for at least 15 minutes.
Keep your eyes open while flushing.
Have yourself checked out at the Health Center.

36. LAB SAFETY ORIENTATION
Chemical on skin:
Flush the affected area for at least 15 minutes
Have yourself checked out at the Health Center.

37. LAB SAFETY ORIENTATION
Ingesting Chemical:
Rinse your mouth out immediately.
Seek medical care or have yourself checked out at the Health Center IMMEDIATELY, if you are on campus.
Do not induce vomiting unless directed to do so by the health care provider.

38. LAB SAFETY ORIENTATION
Inhaling Chemical:
Get out into fresh air.
Have yourself checked out at the Health Center.

39. LAB SAFETY ORIENTATION
Chemical Disposal
When you are done using the chemicals, safely dispose of them as specifically instructed by your Instructor.
Broken glassware Disposal
Dispose of broken glassware in appropriate box.
Do not place broken glassware in the trash.

40. LAB SAFETY ORIENTATION
Glassware Incident
If you get cut from broken glassware, clean out the wound prior to bandaging it.
Have yourself checked out at the Health Center if needed.
If there is blood on the surface, have everyone stay away and call the Instructor IMMEDIATELY.

41. LAB SAFETY ORIENTATION
Handling Sharps
Sharps such as scalpel, needles, etc. can also be hazardous in the lab.
When using scalpel, slow down, do not use it to “saw,” do not put too much pressure on it.

42. LAB SAFETY ORIENTATION
Handling Sharps
Never recap needles.
Dispose of used sharps in “SHARP” disposal container.

43. LAB SAFETY ORIENTATION
Biohazard Safety
Biohazards include: bacteria, viruses, fungi, parasites, or any body fluids.
Pathogens: agents that cause disease.
Bloodborne Pathogens: microorganisms that may be transmitted through direct contact with contaminated blood, bodily fluids, or tissues.

44. LAB SAFETY ORIENTATION
Biohazard Safety
Biohazards include: bacteria, viruses, fungi, parasites, or any body fluids.
Pathogens: agents that cause disease.
Bloodborne Pathogens: microorganisms that may be transmitted through direct contact with contaminated blood, bodily fluids, or tissues.

45. LAB SAFETY ORIENTATION
Biohazardous Material Disposal
Dispose of all biohazardous materials as directed by your instructor.
Do not dispose of them in the trash.

46. LAB SAFETY ORIENTATION
Biohazard Precaution
Treat all human blood & bodily fluids as if they are infected.
Use gloves and other PPE as instructed.
Avoid touching your face with your gloves.

47. LAB SAFETY ORIENTATION
Biohazard Precaution
Place all used sharp objects – both contaminated & non-contaminated (needles, scalpel blades, etc.) in a “SHARPS” container.
Never recap needles.

48. LAB SAFETY ORIENTATION
Leaving the lab - when the lab session is over:
Clean your workstation.
Put all tools and equipment back to their proper place.
Remove gloves and other PPE.
Wash your hands thoroughly before leaving the lab.

49. QUIZ Click on the following link to take the online quiz.
Call Ext for any Questions.
You must pass with 100% correct.
Good luck!
Biology Lab Safety Orientation Quiz

50. Have a safe semester