Presentation is loading. Please wait.

Presentation is loading. Please wait.

Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester.

Published byVictoria Pope Modified over 5 years ago

Similar presentations

Presentation on theme: "Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester."— Presentation transcript:

1 Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester R. Romeu, Marc Sacrista, Erika Barboza, Ana Giménez-Arnau, Antonio Celada, Ramon M. Pujol, Luis F. Santamaria-Babí  Journal of Investigative Dermatology  Volume 133, Issue 4, Pages (April 2013) DOI: /jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Streptococcal extract (SE) induces upregulation of mRNA for ifn-γ, il-17, il-22, and ip-10 in psoriatic cutaneous lymphocyte–associated antigen (CLA)+ T cells cultured with lesional autologous epidermal cells. Culture of freshly isolated circulating CLA+/CLA− CD45RO+CD3+ cells with autologous epidermal cells obtained from lesional psoriatic skin was incubated with or without SE. A time-course analysis of ifn-γ, il-17, il-22, and ip-10 mRNA expression was performed. RNA was taken at the indicated times after activation and determined by real-time PCR. (a) Data presented are from three psoriatic patients and (b) from three controls. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Streptococcal extract (SE) induces IFN-γ and IL-17 production in cutaneous lymphocyte–associated antigen (CLA)+ T cells cultured with autologous lesional epidermal psoriatic cells. Supernatants from cultured memory T cells (CLA+/CLA−) and autologous lesional epidermal psoriatic cells stimulated with SE or with Staphylococcus aureus enterotoxin B (SEB) for controls were analyzed for IFN-γ and IL-17 after 48hours of culture by ELISA. (a–d) Data are presented from five psoriatic patients (p) and three controls (c) as net cytokine production. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Supernatants of streptococcal extract (SE)–activated cutaneous lymphocyte–associated antigen (CLA)+ T cells and epidermal cells generate epidermal hyperplasia in vivo. Intradermal injection of supernatants from CLA+ T cells cultured with lesional epidermal cells activated with SE induces in vivo epidermal hyperplasia in Balb/c mice. Supernatant from day 6 of the culture was injected intradermally in Balb/c mice daily for 4 days, and epidermal hyperplasia was evaluated at day 4. (a–c) Results are shown with histological images of skin biopsies (hematoxylin and eosin) and a comparative graphic of epidermis thickness (in micrometers) after supernatant injections in four mice (P<0.001). Bar=50μm. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Streptococcal extract–induced upregulation of mRNA for ifn-γ, il-17, il-22, ip-10, tnf-α, and il-8 in cultures of cutaneous lymphocyte–associated antigen (CLA)+/CLA− T cells of nonlesional epidermal cells of psoriasis patients. Upregulated gene transcription in the culture 24hours after activation. Data are presented for two patients with nonlesional epidermal cells. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Streptococcal extract–induced upregulation in gene transcription for ifn-γ, il-17, il-22, and ip-10 after 24hours of activation of cutaneous lymphocyte–associated antigen (CLA)+ memory T cells and epidermal cells correlates with antistreptolysin O (ASO) serum levels in psoriatic patients. Patients are identified by number and psoriasis type (C, chronic; G, guttate). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Streptococcal extract (SE)–induced upregulation of IL-17A, IFN-γ, IL-6, and TNF-α in cultures of cutaneous lymphocyte–associated antigen (CLA)+ T cells and epidermal cells depends on HLA class I and HLA class II. Three experiments conducted with patients p18, p19, and p23 are presented. (a) Cytokines induced by SE on CLA+ memory T cells and epidermal cells are inhibited with anti-HLA class I and anti class II blocking antibodies; data are presented as net values. (b) The mean percentage inhibition respect isotype is significantly higher for anti-HLA class II than anti-HLA class I; IL-17A (P<0.0001), IFN-γ (P<0.03), and TNF-α (P<0.001). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester."

Similar presentations

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.
CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,

CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,
Interleukin-10 Downregulates Anti-Microbial Peptide Expression in Atopic Dermatitis  Michael D. Howell, Natalija Novak, Thomas Bieber, Saveria Pastore,

Interleukin-10 Downregulates Anti-Microbial Peptide Expression in Atopic Dermatitis  Michael D. Howell, Natalija Novak, Thomas Bieber, Saveria Pastore,
Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,

Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,
A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis  Weiwen.

A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis  Weiwen.
Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.

Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.
The IL-17A-Producing CD8+ T-Cell Population in Psoriatic Lesional Skin Comprises Mucosa-Associated Invariant T Cells and Conventional T Cells  Marcel.

The IL-17A-Producing CD8+ T-Cell Population in Psoriatic Lesional Skin Comprises Mucosa-Associated Invariant T Cells and Conventional T Cells  Marcel.
Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,

Decreased Expression of Caveolin-1 Contributes to the Pathogenesis of Psoriasiform Dermatitis in Mice  Yukie Yamaguchi, Yuko Watanabe, Tomoya Watanabe,
Juan Liu, Erin Harberts, Antonella Tammaro, Nicholas Girardi, Renata B

Juan Liu, Erin Harberts, Antonella Tammaro, Nicholas Girardi, Renata B
Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms  Lauren Y. Cao, Jin-Sung Chung,

Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms  Lauren Y. Cao, Jin-Sung Chung,
IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Preclinical Studies of a Specific PPARγ Modulator in the Control of Skin Inflammation  Arianna Mastrofrancesco, Daniela Kovacs, Massimiliano Sarra, Emanuela.

Preclinical Studies of a Specific PPARγ Modulator in the Control of Skin Inflammation  Arianna Mastrofrancesco, Daniela Kovacs, Massimiliano Sarra, Emanuela.
Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,
MCPIP1 RNase Is Aberrantly Distributed in Psoriatic Epidermis and Rapidly Induced by IL-17A  Ester Ruiz-Romeu, Marta Ferran, Ana Giménez-Arnau, Beata.

MCPIP1 RNase Is Aberrantly Distributed in Psoriatic Epidermis and Rapidly Induced by IL-17A  Ester Ruiz-Romeu, Marta Ferran, Ana Giménez-Arnau, Beata.
IL-27 Activates Th1-Mediated Responses in Imiquimod-Induced Psoriasis-Like Skin Lesions  Sayaka Shibata, Yayoi Tada, Yoshihide Asano, Koichi Yanaba, Makoto.

IL-27 Activates Th1-Mediated Responses in Imiquimod-Induced Psoriasis-Like Skin Lesions  Sayaka Shibata, Yayoi Tada, Yoshihide Asano, Koichi Yanaba, Makoto.
Α-MSH-Stimulated Tolerogenic Dendritic Cells Induce Functional Regulatory T Cells and Ameliorate Ongoing Skin Inflammation  Matteo Auriemma, Thomas Brzoska,

Α-MSH-Stimulated Tolerogenic Dendritic Cells Induce Functional Regulatory T Cells and Ameliorate Ongoing Skin Inflammation  Matteo Auriemma, Thomas Brzoska,
Effective Treatment of Psoriasis with Narrow-Band UVB Phototherapy Is Linked to Suppression of the IFN and Th17 Pathways  Emőke Rácz, Errol P. Prens,

Effective Treatment of Psoriasis with Narrow-Band UVB Phototherapy Is Linked to Suppression of the IFN and Th17 Pathways  Emőke Rácz, Errol P. Prens,
A Reducing Microenvironment Leads to the Generation of FcεRIhigh Inflammatory Dendritic Epidermal Cells (IDEC)  Natalija Novak, MD, Stefan Kraft, Jörg.

A Reducing Microenvironment Leads to the Generation of FcεRIhigh Inflammatory Dendritic Epidermal Cells (IDEC)  Natalija Novak, MD, Stefan Kraft, Jörg.
Increased Lipocalin-2 Contributes to the Pathogenesis of Psoriasis by Modulating Neutrophil Chemotaxis and Cytokine Secretion  Shuai Shao, Tianyu Cao,

Increased Lipocalin-2 Contributes to the Pathogenesis of Psoriasis by Modulating Neutrophil Chemotaxis and Cytokine Secretion  Shuai Shao, Tianyu Cao,

Similar presentations

Ads by Google