1. Janina Jamasbi et al. BTS 2016;1:131-142
Online Video 4 Kinetics of cross-linked GPVI-Fc binding to collagen under flow GPVI-Fc was incubated with 10% PE-labeled and 90% unlabeled anti-human-Fc Fab2 antibodies in a 1:1 molar ratio for GPVI-Fc cross-linking. The mixtures were added to blood (333nM GPVI-Fc f.c.) containing abciximab before perfusion over collagen at a shear rate of 600/sec for 3min. Binding of PE-labeled GPVI-Fc*Fab2-XL to collagen was recorded by fluorescence video microscopy (1frame/sec) using a 10x objective.
Janina Jamasbi et al. BTS 2016;1:
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