1. DIRECTIONS to follow ASAP:
Read pages silently
On a new sheet of paper, write the title “Proper Use of the Compound Light Microscope”
Write a PHRASE summarizing each of the 10 steps listed (use bullet points…short and sweet!)
You will have 10 minutes to finish silently!

2. Microscope
Basics

3. Ocular lens (Eyepiece)
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Base

4. Where you View the Specimen! Ocular lens (Eyepiece)
Focuses ocular lens
Body Tube
Rotates Objective Lenses
Nosepiece
Arm
Scanning Objective (4x)
Objectives
Low Objective (10x)
Hi Power Objective (40x)
Stage
Hold Slide
Stage Clips
Coarse Adjustment
Moves stage up/down; Use to scan for specimen
Controls light!
Diaphragm
Use in high power for Fine Adjustments
Fine Adjustment
Light
Base

5. Microscope Lenses Eyepiece Low Power (yellow) Objective Lens
Holds Ocular Lens
Magnification of 10X
Scanning (red) Objective Lens
Magnification of 4x
Used for Scanning
Use Coarse Focus Knob
Low Power (yellow) Objective Lens
Magnification of 10X
Used for Scanning
Use Coarse Focus Knob
High Power (blue) Objective Lens
Magnification of 40X
Used for Fine Focusing
Seeing the details
Use Fine Focus Knob ONLY
Do NOT use Coarse Focus Knob

6. The Light Source sends light through the slide.
The Iris Diaphragm regulates the amount of light from the light source that passes through the slide.
The coarse adjustment should only be used in scanning (4x) and low power (10x) objectives. It is used for seeing the slide.
You should use the fine adjustment while in the high power objective. It is used for detailed focus.

7. The stage clips hold the slide and are located on the stage which is moved to view different areas of the slide and specimen.
All of the objectives are held on the nosepiece which rotates to change the power of magnification.
The scanning lens is usually 4x magnification and is used for initial scanning and specimen location on the slide.

8. The low or medium power objective lens is usually 10x magnification and is also used for scanning.
The high power lens is usually 40x or 100x and is used for detailed viewing of a specimen.

9. Brain Pop- Microscopes

10. Finished with Quiz? STAY SEATED…leave dividers UP.
Directions
Get out your Ch. 3-1 Directed reading HW
Study it silently for 5 minutes before the quiz
Finished with Quiz? STAY SEATED…leave dividers UP.

11. (Write calculations & answer)
What’s my power?
To calculate the power of magnification, MULTIPLY: Ocular Lens Power x Objective Lens Power = total magnification
What is the total power of magnification for the Ocular Lens (shown) and the 40x Objective Lens (shown)?
(Write calculations & answer)

12. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

13. SAFETY: Do’s and Don’ts of Microscope Labs!
USE TWO HANDS
Always carry microscopes with one hand on the ARM and the other UNDER the BASE!

14. Do’s and Don’ts
NEVER use Coarse Focus Knob with High Power Lens…it Breaks Slides!
“Ooops, I broke a slide!”
Accidents Happen. Don’t FREAK OUT!
Don’t try to clean it up by yourself!
Raise your hand and a teacher will help you!

15. Cleaning UP! Always do 5 Things! Lower stage Remove slide Wrap cord
Replace plastic cover (7th period)
Put microscope back in designated area with arm out

16. Spooky Cell 4 Stretch me and work me to build more bulk.
Soon you will look like the incredible hulk!

17. Let’s act it out...
1 – PRETEND to Turn on the microscope and then rotate the nosepiece to click the (low power) red-banded objective into place.
2 – Place an IMAGINARY slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers.
Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!
4 – When you are done, turn off the microscope and put up the slides you used.

18. Directions…
Use the bag of cards and match up the microscope part to its function. Work with the person next to you!