1. Haploidentical In Utero Hematopoietic Cell Transplantation Improves Phenotype and Can Induce Tolerance for Postnatal Same-Donor Transplants in the Canine Leukocyte Adhesion Deficiency Model 
William H. Peranteau, Todd E. Heaton, Yu-Chen Gu, Susan W. Volk, Thomas R. Bauer, Keith Alcorn, Laura M. Tuschong, Mark P. Johnson, Dennis D. Hickstein, Alan W. Flake 
Biology of Blood and Marrow Transplantation 
Volume 15, Issue 3, Pages (March 2009)
DOI: /j.bbmt
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
Chimerism in the CLAD model after IUHCT. (A) PB was analyzed monthly by flow cytometry for the CD18 antigen in the CLAD dogs Duke and Billie. Duke's chimerism analysis stopped at 7 months, because he underwent postnatal transplantation at that time. For mulitlineage analysis, PB samples from Billie (B) and Duke (C) were analyzed at the indicated time points by flow cytometry for donor neutrophils, monocytes, B cells, and T cells.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
Engraftment after IUHCT in a female CLAD recipient: correlation of flow cytometry and TaqMan PCR. (A) In Billie, a female CLAD recipient of male donor BM, donor engraftment was confirmed by PCR for the SRY gene. (B) Chimerism was quantified by SRY TaqMan PCR, and levels were found to be similar to those determined by flow cytometry for the CD18 antigen.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

4. Figure 3
Clinical phenotype of chimeric CLAD dogs after IUHCT. (A) The CLAD dogs were monitored and scored using the sum of 7 clinical parameters of temperature, comfort, movement, appearance, behavior, interactive behavior, and vocalization. A score ranging from 0 for normal to 4 for maximal abnormality in each category was assigned at each data point. Data are shown for up to 7 months of life for Duke, at which point he underwent postnatal BMT. Billie continued to experience no CLAD clinical events up to the last time point assessed, at 18 months of life (not represented on the graph). (B) Billie's and Duke's WBC counts were assessed for leukocytosis (> 50,000 cells/μL), which is typical of CLAD-affected dogs.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

5. Figure 4
Recipients of IUHCT were evaluated by in vitro MLR for reactivity against donor cells compared with reactivity against self and unrelated third-party cells. MLR was performed with CFSE staining. (A) Increased cell division/reactivity is indicated by decreased intensity of CFSE staining and a shift of cells from population 1 (P1) to population 3 (P3). (B) The reactivity (or percentage of cells in each population) of recipients to self cells was standardized to 100%, as indicated by the dotted line, and the reactivity of recipients to third-party and prenatal donor cells was compared with this standard. Thus, a bar above the dotted line indicates a larger percentage of cells in that population compared with the percentage of cells in the self-reactive negative control assay.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

6. Figure 5
Donor cell engraftment after postnatal “boosting” BMT. Six dogs underwent postnatal BMT using the same donor as for their prenatal BMT. Two dogs (Terry and Cashmere) did not undergo prenatal BMT and only underwent postnatal BMT, with their mother serving as the donor. Terry and Cashmere demonstrated no donor cell engraftment at any time point analyzed and are thus not represented on the graph.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions

7. Figure 6
Chemotaxis assay to SDF-1α gradient after CD26 inhibition. Canine BM-derived enriched CD34+ cells (A) and whole BM (B) were subjected to CD26 inhibition by incubation with Diprotin A or were unmanipulated (control) and assessed for SDF-1α–mediated chemotaxis after 4 hours of incubation at 37°C in Transwell chemotaxis chambers. As indicated, cells were incubated with Diprotin A at either 37°C or room temperature for 15 minutes before being placed in the chemotaxis chambers. Migration at all SDF-1α concentrations was assessed in triplicate.
Biology of Blood and Marrow Transplantation  , DOI: ( /j.bbmt )
Copyright © 2009 American Society for Blood and Marrow Transplantation Terms and Conditions