1. High-Throughput Nonlinear Optical Microscopy
Peter T.C. So, Elijah Y.S. Yew, Christopher Rowlands 
Biophysical Journal 
Volume 105, Issue 12, Pages (December 2013)
DOI: /j.bpj
Copyright © 2013 The Authors Terms and Conditions

2. Figure 1
Effect of excitation saturation on radial (top) and axial (bottom) point spread functions at different levels of saturation (28). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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3. Figure 2
Schematics of a polygonal scanner-based NLO microscope (29). The 4-F geometry consists of the optics between the polygonal mirror and the galvanometric mirror. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2013 The Authors Terms and Conditions

4. Figure 3
En-face images of the skin of a live mouse, taken by a video-rate SRS microscope, from reference (34). Images of (D) lipid and (E) water highlight the lipid-rich (but water-poor) sebaceous glands, whereas the CH3 stretching vibration imaged in (F) serves to highlight proteins and lipid-rich structures. The arrow indicates a capillary in which individual red blood cells can be seen at higher magnification.
Biophysical Journal  , DOI: ( /j.bpj )
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5. Figure 4
A compact AOD-based NLO fluorescence microscope. The prechirper with the paired prism is for temporal dispersion compensation. Acousto-optical lens (AOL) consisting of four AODs (unlabeled rectangles with shaded interior) with polarizers (P) and half-wave plates (H) to remove the residue-un-diffracted first-order (70). In this setup, the galvanometer mirrors are fixed and scanning is done solely through the AOL. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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6. Figure 5
(Top) An image of calcium-signal propagation along a dendrite of a pyramidal neuron. (Middle) The time-dependent calcium signals at selected locations on the dendrite are plotted. (Bottom) The neuron is patched to allow simultaneous monitoring of voltage signal (8). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2013 The Authors Terms and Conditions

7. Figure 6
Schematic of a multifocal NLO microscope. (a) A lenslet array is used to produce an array of foci that are scanned across the sample in a raster pattern. The signal is detected with an MA-PMT. (Inset) Fluorescent image of a fluorescein sample excited by the multifocal array. (b) An enlarged view of the optical path of the excitation and emission light. (Ti:Sap, titanium sapphire laser; T, telescope; MLA, microlens array; DCM1, dichroic mirror 1; DCM2, dichroic mirror 2; GSM, galvanometric scanning mirror; MA-PMT, multianode PMT; ZP, z-piezo; OL, objective lens; TS, tissue sample; M, motor; DH, microtome; TPB, two-photon barrier filter (71).) To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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8. Figure 7
(a) Scan patterns used with a four-beam multifocal NLO microscope, with representative images of calcium signals, using Fluo-4-AM, from neurons imaged at two levels in the brain. (b) Zero time-lag calcium-signal images of neurons taken at two depths (left) and the corresponding segmented image (right). (c) Raster plot showing firing events from labeled cells identified at the two layers (y axis) as a function of time (x axis). (Red dots) Synchronous correlated firing event; (black dots) firings that are not well correlated. A representative firing pattern for two cells is shown below the raster plot, containing both correlated and uncorrelated events (52). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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9. Figure 8
(a) Principles of spatial and temporal focusing NLO microscopy. (b) Schematics of a temporal-focusing NLO microscope based on the design by Oron et al. (53). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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10. Figure 9
Fast three-dimensionally resolved temporal-focusing wide-field FLIM-PLIM imaging of a sample consisting of human fibroblasts stained with Rhodamine lipid, seeded inside a collagen matrix and treated in buffer containing 1 mM ruthenium-based oxygen sensor. (In sequence from left to right) Intensity image, phosphorescence lifetime-resolved image from phase data, and phosphorescence lifetime-resolved image from modulation data. (Far right) Polar plot of the pixel phosphorescence lifetime-resolved measurements (60). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2013 The Authors Terms and Conditions

11. Figure 10
Schematics of a STEAM white light image cytometer. (3). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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