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Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,

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Presentation on theme: "Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,"— Presentation transcript:

1 Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana, L Seoke, J Hyera, G Matlho, F Vandenbussche, K De Clercq

2 The Study Can unbiased RNASeq produce complete FMDV genome sequences directly from clinical samples? 3 strong positive epithelial samples (CT range 14,63 – 16,18) from Zambia and Namibia

3 Results Unbiased RNASeq produces complete genomes with high average coverage in 2/3 samples limited gap closure (Sanger sequencing) needed in 3rd sample due to low coverage (limited % of FMDV reads). Potential for unbiased detection of co-infections If only targeting FMDV, targeted approaches may produce better coverage. e.g. Logan G, Freimanis GL, King DJ, Valdazo-Gonzalez B, Bachanek-Bankowska K, Sanderson ND, Knowles NJ, King DP, Cottam EM. (2014) BMC Genomics 15:828

4 Conclusion These data demonstrate the feasibility of direct sequencing of complete FMDV coding sequences from samples from symptomatic animals The complete genome sequences of O/ZAM14/2010, SAT1/NAM01/2010 and SAT2/ZAM18/2009 were obtained

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