1. Fertility preservation and refreezing of transplanted ovarian tissue—a potential new way of managing patients with low risk of malignant cell recurrence 
Stine Gry Kristensen, Ph.D., Veronica Giorgione, M.D., Peter Humaidan, M.D., Birgit Alsbjerg, M.D., Anne-Mette Bay Bjørn, M.D., Erik Ernst, M.D., Claus Yding Andersen, D.M.Sc. 
Fertility and Sterility 
Volume 107, Issue 5, Pages (May 2017)
DOI: /j.fertnstert
Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Frozen and grafted ovarian tissue. (A) Ovarian tissue frozen in (B) Recovered ovarian grafts extracted in (C) Representative histologic image of healthy primordial follicles in the fresh ovarian tissue. (D) Representative histologic images of healthy pre-antral stages of follicles in the recovered grafted ovarian tissue. Scale bars = 50 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
(A) Follicular distribution in fresh ovarian tissue and grafted ovarian tissue. Follicles were classified and counted in the pre-cryopreservation control sample (fresh ovarian biopsy taken on the day of cryopreservation in 2003) and in the recovered grafted tissue 3.3 years and 4.5 years after transplantations. Total follicles per biopsy as well as percentages of primordial, primary, and secondary follicles are presented. The percentage of primordial follicles after 3.3 years of grafting (20.5%) was markedly lower compared with the pre-cryopreservation control sample (93%). The percentages of primary and secondary follicles were also notably higher in the post-grafted tissue (48.0% and 31.5%, respectively) compared with the pre-cryopreservation control sample (5.6% and 1.4%, respectively). (B) Follicular health in fresh ovarian tissue compared with grafted ovarian tissue several years after transplantation. Bars depict the percentage of healthy and atretic follicles within the three stages of pre-antral follicles—primordial, primary, and secondary follicles—in the pre-cryopreservation control tissue and in the post-grafted ovarian tissue recovered in August Total numbers of follicles are depicted in parentheses.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Follicular survival in refrozen ovarian tissue after 4 weeks of transplantation to an immunodeficient mouse. (A) Two pieces of refrozen ovarian grafted tissue just after thawing. (B) Grafts after 4 weeks of xenografting showing nice revascularization of both grafts. Scale bar = 250 μm. (C) Surviving pre-antral follicles within the grafts. Scale bar = 50 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions