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Development of a quantitative immunofluorescence assay for detection of Stenotrophomonas maltophilia antibodies in patients with cystic fibrosis  P. Gonçalves.

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Presentation on theme: "Development of a quantitative immunofluorescence assay for detection of Stenotrophomonas maltophilia antibodies in patients with cystic fibrosis  P. Gonçalves."— Presentation transcript:

1 Development of a quantitative immunofluorescence assay for detection of Stenotrophomonas maltophilia antibodies in patients with cystic fibrosis  P. Gonçalves Vidigal, D. Schmidt, F. Stehling, U. Mellies, E. Steinmann, J. Buer, P.-M. Rath, J. Steinmann  Journal of Cystic Fibrosis  Volume 12, Issue 6, Pages (December 2013) DOI: /j.jcf Copyright © 2013 European Cystic Fibrosis Society. Terms and Conditions

2 Fig. 1 A) Antibody levels of S. maltophilia (Sm) whole cell in serum samples from healthy subjects (healthy), CF patients with chronic (CF chronic Sm), intermittent (CF intermittent Sm), and never Sm without P. aeruginosa (CF never Sm/Pa). Antibody levels are reported as titres. P values< by non-parametric test were considered significantly different. B) Comparison of antibody titre between CF “never Sm/Pa” and CF never Sm but chronic Pa. Antibody titres did not differed between the groups (P=0.1898). Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2013 European Cystic Fibrosis Society. Terms and Conditions

3 Fig. 2 Receiver operating curve (ROC) for IFA detecting serum antibodies against Sm whole cell. ROC data (area=0.8815; std. error=0.052; P=0.0002) of CF patients never colonised S. maltophilia/P. aeruginosa (Sm/Pa) versus CF patients with chronic S. maltophilia (Sm). Journal of Cystic Fibrosis  , DOI: ( /j.jcf ) Copyright © 2013 European Cystic Fibrosis Society. Terms and Conditions


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