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K. Schulze Johann, M. Schürenkamp, U. Sibbing, C. Lischka, H

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Presentation on theme: "K. Schulze Johann, M. Schürenkamp, U. Sibbing, C. Lischka, H"— Presentation transcript:

1 Linear-after-the-exponential (LATE)-PCR: Improved asymmetric PCR for quantitative DNA-analysis 
K. Schulze Johann, M. Schürenkamp, U. Sibbing, C. Lischka, H. Pfeiffer, M. Vennemann  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e659-e661 (December 2015) DOI: /j.fsigss Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Comparison of conventional PCR (blue) and LATE-PCR of TH01 (green). The plateau phase is clearly visible for conventional PCR while the LATE-PCR shows constant increase of the PCR product amount up to 70 cycles. Forensic Science International: Genetics Supplement Series 2015 5, e659-e661DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Comparison of different DNA-concentrations in LATE-PCR of TH01. A clear relationship between end point quantities and target quantities seem to exist; however, further optimization is needed to improve the correlation coefficient. Forensic Science International: Genetics Supplement Series 2015 5, e659-e661DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions


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