1. Volume 9, Issue 2, Pages 581-590 (October 2014)
Development of the Fetal Bone Marrow Niche and Regulation of HSC Quiescence and Homing Ability by Emerging Osteolineage Cells 
Süleyman Coşkun, Hsu Chao, Hema Vasavada, Kartoosh Heydari, Naomi Gonzales, Xin Zhou, Benoit de Crombrugghe, Karen K. Hirschi 
Cell Reports 
Volume 9, Issue 2, Pages (October 2014)
DOI: /j.celrep
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2. Cell Reports 2014 9, 581-590DOI: (10.1016/j.celrep.2014.09.013)
Copyright © 2014 The Authors Terms and Conditions

3. Figure 1
Fetal Bone Is Vascularized within the Middle Region at E16.5 when Hematopoietic Stem/Progenitor Cell Activity Is Initially Detected
(A) Mouse E15.5–E17.5 fetuses were injected with 100 μl Dextran-FITC (70 kDa) via the superficial temporal vein to reveal functional blood vessels. Fetal femurs were avascular at E15.5, but perfused by E16.5, specifically in the middle regions. Longitudinal bone cross-section, green: Dextran-FITC, blue: nuclear DAPI. Scale bar, 100 μm.
(B) Composition of fetal bone was revealed via alizarin red (mineralized tissue) and alcian blue (cartilage) staining. Calcification was apparent by E16.5 in the middle regions and present throughout the tissue by E17.5.
(C) At E16.5, Col1a1-positive mature osteoblasts were detected at the periosteum and throughout the bone at E17.5. Longitudinal bone cross-section; scale bar, 100 μm.
(D) HSPC activity was first detected at E16.5 in the middle regions of bone. Multilineage colonies containing granulocyte, erythrocyte, monocyte, and megakaryocyte (CFU-GEMM) were scored from 20,000 cells/well for each embryonic time point by Methocult assay. Data represent mean ± SEM (n = 3).
Cell Reports 2014 9, DOI: ( /j.celrep )
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4. Figure 2
Fetal Bone Marrow Hematopoietic Activity Is Restricted to the KSL Population, which Is More Actively Proliferating Than Adult Bone Marrow KSL and Gives Rise to All Blood Lineages
(A) 100 KSL and 1000 non-KSL cells from fetal bone marrow were sorted and cultured in Methocult; only KSL cells exhibited multilineage colony-forming activity. Data represent mean ± SEM (n = 3); p = (E16.5), p = (E17.5), and p = (E18.5).
(B) Cell-cycle distribution of the fetal and adult bone marrow KSL cells was revealed using Pyronin Y/Hoechst dyes and flow cytometry. KSL cells were more actively proliferating in fetal versus adult bone marrow. Data represent mean ± SEM (n = 3).
(C) Engraftment levels were determined at 4, 12, and 20 weeks posttransplantation by analyzing peripheral blood CD45.2 (donor) and CD45.1 (recipient) levels. LT-HSCs were first present at E16.5 and showed engraftment levels up to 35% at 20 weeks posttransplant. By E17.5, engraftment level was comparable to adult bone marrow HSCs. Data represent mean ± SEM (n = 3). Differences between E16.5 and either E17.5 or adult were significant; p = (week 4), p = (week 12), and p = (week 20).
(D) Lineage analysis revealed fetal bone marrow cells (E16.5–E17.5) gave rise to all blood lineages in vivo. Data represent mean ± SEM (n = 3).
Cell Reports 2014 9, DOI: ( /j.celrep )
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5. Figure 3
Osx−/− Fetal Bone Marrow Cells Form Multilineage CFU-GEMM Colonies In Vitro but Fail to Repopulate Transplanted Recipients
(A) Osx−/− fetal long bones lack osteoblasts and have structural defects compared to wild-type (WT); however, they have a normally vascularized marrow, as revealed by isolectin B4-FITC staining. Scale bar, 20 μm.
(B) Representative images of CFU-GEMM and CFU-GM colonies formed from E16.5 and E17.5 Osx−/− fetal bone marrow cells, similar to E17.5 WT fetal bone marrow cells.
(C) A total of 400,000 WT and Osx−/− WBM cells at E17.5 were transplanted into sublethally irradiated neonates, and engraftment levels were determined by analyzing recipients’ peripheral blood up to 20 weeks posttransplant; E17.5 Osx−/− WBM cells did not engraft. Data represent mean ± SEM (n = 3); p = (week 4), p = (week 12), and p = 0.03 (week 20).
(D) WT and Osx−/−-derived E17.5 fetal liver cells repopulated transplant recipients’ peripheral blood in vivo. Data represent mean ± SEM (n = 3); p = (week 4), p = (week12) and p = (week 20).
Cell Reports 2014 9, DOI: ( /j.celrep )
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6. Figure 4
Fetal Bone Marrow Cells Derived from Osx−/− Mutants Exhibit a Homing Defect
(A) P0–P2 recipient neonates were euthanized 24 hr posttransplantation, and bone marrow and liver cells were analyzed with flow cytometry for donor/recipient cell ratios.
(B) Fetal liver cells from E17.5 WT and Osx−/− mutants exhibited similar homing to liver and bone marrow of WT recipients.
(C) In contrast, fetal WBM cells derived from E17.5 Osx−/− mutants showed significantly reduced homing compared to WT. Data represent mean ± SEM (n = 3).
Cell Reports 2014 9, DOI: ( /j.celrep )
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7. Figure 5
Gene Expression Studies Revealed that Genes Specific to the Bone Marrow Niche and HSPCs Are Dysregulated in Osterix Mutants
(A) Gene expression analysis (via qPCR) was performed on E17.5 WT and Osx−/− WBM cells. Osx expression was ∼40% decreased in Osx+/− fetuses and absent in Osx−/− mutants.
(B) N-cadherin (cdh2) and osteopontin (opn) were significantly downregulated in Osx−/− mutants, whereas Cxcl12, kitL (SCF), nestin, vascular-endothelial cell adhesion protein, VE-cadherin (cdh5), vascular-endothelial growth factor receptor 2 (vegfr2), and integrin subunits β1 (itgb1) and α5 (itga5) were significantly upregulated compared to WT (∗p ≤ 0.01, ∗∗p ≤ 0.001, and ∗∗∗p ≤ ). β-actin gene expression was used as an internal control. For each gene of interest, triplicates from four different cDNA samples were analyzed. Data represent mean ± SEM (n = 4; triplicates for each sample).
(C and D) KSL cells were isolated from the hindlimbs of E17.5 WT and Osx−/− mutants and analyzed in ten-cell pools. In Osx−/− KSL cells, the expression of CD38, p21, p53, as well as Tie2 and CD44, trended toward higher than wild-type littermates, whereas expression of Hif1α and VegfA trended toward lower. β-Actin served as an internal control. Statistical analyses were done using the two-tailed unpaired Student’s t test. Data represent mean ± SEM (n = 4); p = 0.30 (CD38), p = 0.15 (p21), p = 0.30 (p53), p = 0.17 (Tie-2), p = 0.28 (CD44), p = 0.44 (Hif1a), p = 0.33 (Vegfa), p = 0.5 (itgb1), p = 0.79 (Selpg), p = 0.42 (itga4), and p = 0.29 (notch1).
Cell Reports 2014 9, DOI: ( /j.celrep )
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8. Figure 6
Working Model
In wild-type (WT) fetal long bones (femur), adult repopulating HSCs are first present at E16.5, coincident with vascular perfusion, localized to vascularized regions of bone, and highly proliferative relative to adult HSCs. Osteoblast progenitors are also present within fetal bone at this stage but appear to be restricted to the periosteum. From E17.5 onward, HSCs are also detected within the proximal and distal ends of bone that are fully vascularized and contain mature osteoblasts that are thought to regulate HSC quiescence. In Osx−/− fetal bone marrow, osteoblasts and osteolineage cells do not develop, and there is increased expression of vascular niche associated genes, including VE-cadherin, Vegfr2, KitL, and cxcl12, as well as decreased expression of N-cadherin and osteopontin, which suggests increased representation of vascular endothelial cells in this microenvironment in osterix-null mutants. HSPC phenotype and function are also altered in the osteolineage cell-deficient microenvironment. Although KSL cells are present in equal numbers compared to WT, and exhibit similar apoptosis levels, KSL cells isolated from Osx−/− fetal bone marrow exhibited dysregulated cell-cycle progression and defective homing ability. These data suggest that osteolineage cells in fetal bone marrow play a critical role in establishing and sustaining LT-HSC phenotype and function during development.
Cell Reports 2014 9, DOI: ( /j.celrep )
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