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Assisted oocyte activation following ICSI fertilization failure

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Presentation on theme: "Assisted oocyte activation following ICSI fertilization failure"— Presentation transcript:

1 Assisted oocyte activation following ICSI fertilization failure
Frauke Vanden Meerschaut, Dimitra Nikiforaki, Björn Heindryckx, Petra De Sutter  Reproductive BioMedicine Online  Volume 28, Issue 5, Pages (May 2014) DOI: /j.rbmo Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 Calcium oscillating pattern recorded following mouse ICSI. The first calcium rise, partially visible at time point 0, is provoked by the ICSI procedure itself. A second large calcium spike is seen within 10–15min following ICSI and this is followed by a series of calcium oscillations. AU=arbitrary units (recorded by F Vanden Meerschaut, Physiology Group, Department of Basic Medical Sciences, Ghent University). Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Schematic overview of the mouse oocyte activation test (MOAT). Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

4 Figure 3 Calcium response in in-vitro matured human oocytes during assisted oocyte activation (AOA) using ionomycin, as described by Rybouchkin et al. (1997). The first calcium rise is provoked by the ICSI procedure combined with calcium chloride injection (black arrow). The second and third calcium rises are provoked by the first and second ionomycin exposures, respectively (blue arrows). The insert represents the first 1.5h of calcium measuring. AU=arbitrary units (recorded by F Vanden Meerschaut, Physiology Group, Department of Basic Medical Sciences, Ghent University). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions


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