1. sgSNP 377 Contig21552 sgSNP 247 Contig11105 sgSNP 1433 Contig10821
Reference reaction
Reaction with allele 1
Reaction with allele 2
2.0
1.5
1.0
sgSNP 377 Contig21552
0.5
65.0
75.0
80.0
85.0
90.0
95.0
70.0
0.8
0.6
0.4
0.2
sgSNP 247
Contig11105
0.0
65.0
75.0
80.0
85.0
90.0
95.0
70.0
1.6
1.4
1.2
1.0
0.8
0.6
sgSNP 1433
Contig10821
0.4
0.2
65.0
70.0
75.0
80.0
85.0
90.0
95.0
1.5
1.3
1.1
0.9
0.7
sgSNP 193
Contig10284
0.5
0.3
0.1
65.0
70.0
75.0
80.0
85.0
90.0
95.0
1.6
1.4
1.2
1.0
0.8
0.6
0.4
sgSNP 521
Contig 17875
0.2
65.0
70.0
75.0
80.0
85.0
90.0
95.0
65.0
75.0
80.0
85.0
90.0
95.0
70.0
1.4
1.2
1.0
0.6
0.4
0.8
0.2
sgSNP 1297
Contig18072
Supplemental Figure S6. Amplification plot (left) and melting curve (right) of sgSNPs by qPCR. The amplification graphics show the ΔRn during the cycles reaction to different reactions (reference reaction, reaction with allele 1 and reaction with allele 2). The melting curve graphic shows the representation of derivative reporter (-Rn) during increase of temperature and the formation of only one peak showing that the reaction had specific amplification.