1. Transport in Plants
Chapter 36

2. I) Transport at the Cellular Level
A) Selective Permeability 1) Diffusion: movement from [high] to [low] 2) Passive transport: movement without the use of ATP

3. 3) Transport proteins: channels that increase the rate at which solutes can diffuse across the membrane

4. 4) Active transport: use of ATP to pump solutes against the electrochemical gradient.

5. 5) Gated channels a) open or close based on environmental stimuli

6. B) Central Role of Proton Pumps
1) These are transport proteins that pumps H+ ions out of the cell a) creates a H+ gradient b) creates a membrane potential b1) now more + outside of the cell

7. b2) – charge in the cell helps K+ diffuse into the cell.

8. 2) Cotransport: diffusion of H back into the cell through t-port protein helps bring others in against their gradient a) NO3- b)Sucrose

9. C) Water transport
1) Water potential(ψ) a) combined effects of solute concentration and pressure from presence of cell wall a1) water moves from higher potential to lower potential

10. b) cell wall creates a pressure that lets cell have solutes in it but not keep taking in water.

11. c) flaccid: low pressure, cell is not firm d) plasmolyze: water leaves cell, cell shrinks e) turgor pressure: water goes in cell and pushes against cell wall f) turgid: cell is full of water and is firm

12. D) Aquaporins
1) transport proteins that allow the passive movement of water across the cell membrane a) affect the rate at which water can flow

13. E) Role of Vacuole and other compartments
1) Tonoplast: membrane of vacuole that controls movement of materials between vacuole(cell sap) and cytoplasm a) proton pumps draw H+ into vacuole to help keep H+ low in the cytosol

14. 2) Symplast
a) connection of cells by plasmodesmata a1) creates one continuous cytoplasm

15. 3) apoplast
a) connection of cell walls that create channels between cells

16. II) Absorption by Roots
A)surface area 1) root hairs: extensions of membrane of epidermis a) water diffuses in, travels along symplast or apoplast to stele b) minerals actively transported in, helping to keep water concentration low in root

17. 2) mycorrhizae
b) symbiosis between roots and hyphae (the “body” of a fungi) b1) root gets nutrients, water, and other minerals b2) fungus gets carbs

18. III) Transport in Xylem
1) water moves in and out of cells by osmosis, but requires pressure (both positive and negative)to move up a plant = bulk flow

19. A) Pushing xylem sap
1) Root pressure: a) endodermis and casparian strip keeps minerals in stele b) causes high water potential to cause water to diffuse into stele c) this pushes water up plant

20. d) guttation
d1) droplets formed when water is forced out of leaves at night from root pressure – only works over short distances

21. B) Pulling Xylem Sap: the transpiration-cohesion-tension model
1) Transpirational pull a) water evaporates from stomate b) this creates an area of lower water potential, allowing water to be pulled out of the xylem

22. 2) cohesion and adhesion a) water sticks to sides of xylem, keeping it from sliding down xylem b) cohesion helps pull water up length of xylem

23. IV) Control of Transpiration
A) The photosynthesis-transpiration compromise 1) Need CO2 in through stomata, but water is also lost. transpiration video

24. 2) transpiration to photosynthesis ratio a) C3 plants have a 600g of water to 1g of CO2 ratio b) C4 plants have a 300g of water to 1g of CO2 ration

25. 3) transpiration also brings minerals to leaves and cools them off

26. A) How stomata open and close
1) when guard cells are more turgid, the stomate is more open. This happens when they actively take in K+. a) this causes a lower water potential inside, and water then diffuses in.

27. 2) Stomates open in morning when blue light receptors in the membrane are activated and start the H+ pump.

28. 3) Stomates open from lack of CO2 because of increase in photosynthesis

29. 4) 3rd cue for opening in the morning is based on circadian rhythms
4) 3rd cue for opening in the morning is based on circadian rhythms.(intervals of about 24hrs)

30. 5) closing during the day caused by stress a) loss of turgor b) abscisic acid (hormone) is released by mesophyll cells when there is a lack of water

31. B) Xerophyte adaptations
1) plants living in arid climates have to severely limit loss of water by transpiration a) small thick leaves b) thick cuticle c) stomata on bottom of leaf d) store water in stem(cacti)

32. e) Succulents: do photosynthesis by crassulacean acid metabolism(CAM) e1) incorporate CO2 into organic acids at night. During day stomates close and organic acids are broken down to put CO2 into C3 pathway for photosynthesis

33. V) Translocation of Phloem Sap
A) Move sap from sugar source to sugar sink 1) Source: sugar is being made by photosynthesis or starch breakdown. 2) Sink: part of plant using or storing sugar

34. B) Phloem loading and unloading
1) Depending on plant, will move along apoplast and/or the symplast. 2) transfer cells: modified companion cells with ingrowths in walls to increase surface area for transfer between symplast and apoplast

35. C) Pressure flow moves phloem sap
Steps:
1) high [sugar] at source causes water to come in by osmosis
2) transport of sugar out of sieve tube at sink causes water to diffuse out of sieve tube.
3) this creates a difference in water potential, driving sap toward sink