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Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections N.J. Gadsby, M.P. McHugh, C.D. Russell, H. Mark, A. Conway Morris, I.F. Laurenson, A.T. Hill, K.E. Templeton Clinical Microbiology and Infection Volume 21, Issue 8, Pages 788.e1-788.e13 (August 2015) DOI: /j.cmi Copyright © 2015 The Authors Terms and Conditions
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Fig. 1 Distribution of Cq values in triple unequal mixture (n = 6) and single (n = 1) plasmid positive control material for each assay target at three concentrations (200, 20 000 and 200 000 gene copies/reaction). Clinical Microbiology and Infection , 788.e1-788.e13DOI: ( /j.cmi ) Copyright © 2015 The Authors Terms and Conditions
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Fig. 2 Bacterial loads calculated by multiplex real-time PCR (mRT-PCR) in culture-positive (n = 20) and culture-negative (n = 20) sputum specimens from patients with pneumonia. Ab, Acinetobacter baumannii; Ec, Escherichia coli; Hi, Haemophilus influenzae; Kp, Klebsiella pneumoniae; Mc, Moraxella catarrhalis; Pa, Pseudomonas aeruginosa; Sa, Staphylococcus aureus; Sp, Streptococcus pneumoniae. Clinical Microbiology and Infection , 788.e1-788.e13DOI: ( /j.cmi ) Copyright © 2015 The Authors Terms and Conditions
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