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Internal validation of Tecan robots (Freedom EVO® 150 and 75) for PCR and capillary electrophoresis setup  Nadja V. Morf, Andrea Sulzer, Adelgunde Kratzer,

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Presentation on theme: "Internal validation of Tecan robots (Freedom EVO® 150 and 75) for PCR and capillary electrophoresis setup  Nadja V. Morf, Andrea Sulzer, Adelgunde Kratzer,"— Presentation transcript:

1 Internal validation of Tecan robots (Freedom EVO® 150 and 75) for PCR and capillary electrophoresis setup  Nadja V. Morf, Andrea Sulzer, Adelgunde Kratzer, Walter Bär  Forensic Science International: Genetics Supplement Series  Volume 3, Issue 1, Pages e89-e90 (December 2011) DOI: /j.fsigss Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Mean sample peak height of the 16 different samples; measured five times to test the repeatability of the CE setup robot. The mean standard deviation of the mean peak height of the samples pipetted by the robot (A, mean SD: 59.7) was lower than the one of the hand-pipetted samples (B, mean SD: 100.7). Forensic Science International: Genetics Supplement Series 2011 3, e89-e90DOI: ( /j.fsigss ) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Influence of different PCR product volumes (1, 1.2 or 1.4μl) on the mean sample peak height (A) and mean size standard peak height (B). The volume of PCR product does not influence the mean sample peak height (A). 1μl of PCR product pipetted by the robot produces a similar mean size standard peak height as 1.2μl PCR product pipetted by hand pipettes (B). Forensic Science International: Genetics Supplement Series 2011 3, e89-e90DOI: ( /j.fsigss ) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions


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