1. PATHOLOGY PRACTICALS-II LEUKEMIAS & MULTIPLE MYELOMA

2. MYELOBLAST
10-18 um diameter
Round to oval nucleus
2-5 nucleoli in the nucleus
Chromatin in dispersed
Basophilic cytoplasm at time vacuolated
Auer rods seen in the cytoplasm
MPO POSITIVE
CD13, CD14, CD15 and CD33 antigen POSITIVE

3. NORMOBLASTS
MYELOBLAST 3

4. Myeloblasts in AML Myeloblasts with Auer rods4

5. MYELOBLAST WITH AUER ROD5

6. LYMPHOBLAST
10-18 um
Cytoplasm is agranular
Cytoplasm is moderately basophilic
Chromatin is coarse
1-2 nucleoli
MPO NEGATVE
CD10.CD19,CD20 and CD22 antigen positive

7. MYELOBLASTS LYMPHOBLASTS7

8. CHRONIC MYELOID LEUKEMIA
Peripheral blood film shows-
Segmented neutrophils
Myelocytes, few myeloblasts (<10%)
Basophilia
Eosinophilia
(Bimodal population of myeloid series of cells)

9. CML- peripheral blood smear show immature myeloid cells,
band forms and segmented mature neutrophils 9

10. CML- peripheral blood smear shows myelocyte metamyelocyte,
basophil and eosinophils 10

11. CHRONIC LYMPHOID LEUKEMIA
Peripheral blood film shows-
Absolute lymphocytosis (mature lymphocytes) upto 90%
Smudge cells/ smear cells

12. CLL 12

13. Markedly elevated in peripheral blood smear in CLL
MATURE LYMPHOCYTES
Markedly elevated in peripheral blood smear in CLL 13

14. HAIRY CELL LEUKEMIA Peripheral blood film shows-
Abnormal mononuclear cells with hairy projections from the cell membrane

15. 15

16. HAIRY CELL LEUKEMIA
Malignant cells are positive for TRAP (tartrate resistant acid phophatase)

17. HAIRY CELL TRAP TEST +VE
HAIRY CELL LEUKAEMIA
HAIRY CELL TRAP TEST +VE 17

18. Reed Sternberg cell
Bilobed nuclei/owl-eyed nuclei with prominent inclusion like eosinophilic nucleoli.
Abundant blue cytoplasm
Surrounded by lymphocytes
Seen in Hodgkin lymphoma
CD 15 and CD 30 positive.
Variants- lacunar histiocyte, popcorn cell

19. * * Burkitt’s lymphoma 7 yearAfrican child with swelling of jaw
Tumor is ulcerated and draining
Burkitt’s lymphoma
Monotonous population
of malignant lymphoid cells.
Starry-sky pattern is due to tingle body laden
macrophages (*) (containing
the apoptotic tumor cell) * *

20. MULTIPLE MYELOMA The bone marrow shows:
Plasma cells with basophilic cytoplasm, eccentric nucleus and coarse nuclear chromatin