1. Padova sequencing contribution:
100,000 Fosmid ends
4-5 runs 454-Titanium
2 slides SOLiD (10Kb inserts, 50x2 bases)‏

2. Data production 2

4. Mapping SOLiD reads on 454 contigs

5. Sorting out the links

6. Counting links to produce SOLID arches
4 possible ways to merge 2 fragments
Count all possible merging betweenall the possible pairs of contigs

7. Building a path to order the contigs
List of links (arches)‏
1: 9, 12
2: 6,18
3: 17, 20
4: 5
5: 4, 15
6: 2, 16
7: 14, 10
8: 11, 19
9: 1, 16
10: 7
11: 8, 15
12: 1, 17
13: 14, 20
14: 7, 13
15: 5, 11
16: 6, 9
17: 3, 12
18: 2, 19
19: 8, 18
20: 3, 13
From the above list it should be easily produced the following scaffold:
– 20 – 13 – 14 –