1. The Peripheral Clock Regulates Human Pigmentation
Jonathan A. Hardman, Desmond J. Tobin, Iain S. Haslam, Nilofer Farjo, Bessam Farjo, Yusur Al-Nuaimi, Benedetto Grimaldi, Ralf Paus 
Journal of Investigative Dermatology 
Volume 135, Issue 4, Pages (April 2015)
DOI: /jid
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Partial silencing of BMAL1 or PER1 increases melanin content of human hair follicle (HF) and melanosome number. In both the PER1 (a) and BMAL1 (b) knockdown groups, there was a higher melanin content in HFs (c; scale=50 μm; n=22). Subsequently, the number of melanosomes in melanocytes and keratinocytes was determined from high-resolution light microscopy (d, e; × 1,000 magnification; n=9, three patients; f, g; n=9, three patients) finding that PER1 silencing increased the melanosome number in melanocytes and keratinocytes at both time points. BMAL1 silencing increased the melanosome number in both melanocytes and keratinocytes at 24 hours (Student’s t-test, *P<0.05, **P<0.01, ***P<0.001, error bars are ±SEM). DP, dermal papilla.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Silencing core clock members increases gp100 immunoreactivity, melanocyte dendricity, and tyrosinase activity in human HFs. (a, e) The melanocyte marker gp100 was enriched in the siPER1 group at 24 hours and both knockdown groups 4 days post knockdown. (b, e) gp100 analysis demonstrated that silencing PER1 increases melanocyte number at both time points, yet BMAL1 silencing decreased melanocyte number at 24 hours, recovering by day 4, and increases dendricity for both knockdown groups when compared to the scrambled oligo control at 24 hours, and at day 4 for the BMAL1 knockdown group (d). (c, f) Finally, tyrosinase activity in situ was significantly increased in both knockdown groups (n=27; Student’s t-test, *P<0.05, **P<0.01, ***P<0.001, error bars and ±SEM from three patients). Arrowheads depict immunoreactive melanocytes.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Silencing of core clock genes increases tyrosinase transcription and MITF phosphorylation. Quantitative real-time reverse-transcriptase–PCR was performed on clock members (PER1, BMAL1, and CRY1) and key genes involved in melanogenesis (gp100, MITF, tyrosinase (TYR), TYRP1,2). Significant transcript level changes were observed for tyrosinase in both knockdown groups (a), with PER1 silencing also increasing TYRP1. Although MITF transcript levels did not change, the levels of phosphorylated MITF (MITF-p) increased in the PER1 knockdown group (b, c). As BMAL1 leads to the transcription of PER1, the role of BMAL1 silencing on PER1 protein and mRNA was assessed finding that both were reduced (d, e; Student’s t-test, *P<0.05, **P<0.01, ***P<0.001, error bars are ±SEM, n=15 from three patients).
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Knockdown of clock proteins affects human skin pigmentation and cultured primary epidermal melanocytes. To identify whether these observations are specific to HF melanocytes, clock members PER1 and BMAL1 were silenced in skin biopsies and cultured primary melanocytes. There was a significant increase in melanin content in the skin (a) and gp100 immunoreactivity in both knockdown groups (b); however, there was no change in primary melanocytes (e). Tyrosinase activity was increased in both the skin (c) and primary melanocytes (e) in the knockdown. Similarly, MITF-P was significantly increased (f). (g) After knockdown in primary melanocytes, there was also an increase of TYRP1 in BMAL1-silenced HFs with TYRP1,2 increasing in the PER1 knockdown (Student’s t-test, **P<0.01, ***P<0.001, error bars are ±SEM, n=6). Arrowheads depict melanocytes in situ. AU, arbitrary units; DEJ, dermal epidermal junction; SC, stratum corneum.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions