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Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

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Presentation on theme: "Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites."— Presentation transcript:

1 Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins
Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins A–H Endogenous MOSPD2 (green) staining in HeLa cells expressing Flag‐STARD3 (A), Flag‐STARD3 FA/YA (B), GFP‐ORP1L (C), GFP‐ORP1L FA/YA (D), Flag‐STARD11 (E), Flag‐STARD11 D324A (F), HA‐PTPIP51 (G), or HA‐PTPIP51 ΔFFAT (H) (magenta). Endogenous MOSPD2 was recruited around endosomes, Golgi, and mitochondria by STARD3 and ORP1L, STARD11 and PTPIP51, respectively. FFAT‐deficient STARD3, ORP1L, STARD11, and PTPIP51 mutants did not recruit endogenous MOSPD2. Note that in agreement with immunoprecipitation assays (Fig 5F), PTPIP51 ΔFFAT retained a partial ability to recruit MOSPD2. The subpanels on the right are higher magnification (3.5×) images of the area outlined in white. The Overlay panel shows merged green and magenta images. The Coloc panel displays a co‐localization mask on which pixels where the green and the magenta channels co‐localize are shown in white. Right: Linescan analyses with fluorescence intensities of the green and magenta channels along the white arrow shown on the subpanel Overlay. Black rectangles indicate the positions of late endosomes (E), Golgi stacks (G), and mitochondria (M). Scale bars: 10 μm. Thomas Di Mattia et al. EMBO Rep. 2018;19:e45453 © as stated in the article, figure or figure legend

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