1. Determination of succinylcholine in plasma by high-pressure liquid chromatography with electrochemical detection 
N.I. Pitts, D. Deftereos, G. Mitchell 
British Journal of Anaesthesia 
Volume 85, Issue 4, Pages (October 2000)
DOI: /bja/
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

2. Fig 1
Reaction 1: Hydrolysis of succinylcholine (Succ) by butyrylcholinesterase (BuChE). The first step yields succinylmonocholine (SmCh) and choline. SmCh is then hydrolysed to yield choline and succinate. Reaction 2: Hydrolysis of acetylcholine and choline by acetylcholinesterase (AchE) and choline oxidase, respectively, in the Bioanalytical Systems immobilized enzyme reactor (see text for details).
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

3. Fig 2
Post-column enzyme reactor and analytical column sequence. The trapping column removes physostigmine so that it does not poison the enzyme reactor.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

4. Fig 3
Amount of choline formed from succinylcholine hydrolysis (where 100% represents the amount expected from complete hydrolysis of succinylcholine in the first reaction step). A 0.1 ml sample in 0.5 ml phosphate buffer was incubated at 37°C under the following conditions: 100 μM succinylcholine standard with 20 or 200 mU BuChE, 124 nmol succinylcholine per ml of plasma with 200 mU BuChE in heat-inactivated or fresh plasma or with 10−5 M physostigmine.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

5. Fig 4
Amount of choline formed from succinylmoncholine (SmCh) and succinylcholine (Succ) hydrolysis (where 100% represents the amount expected from complete hydrolysis of succinylcholine in the first reaction step). A 0.1 ml fresh plasma sample in 0.5 ml 500 mM phosphate buffer was incubated with 200 mU BuChE at 37°C with succinylmonocholine at 50 μM or succinylcholine at 129 μM or both together.
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions

6. Fig 5
Concentration of succinylcholine in plasma. Succinylcholine (10.5 mg) was added to a 500 ml pool of donor blood. Duplicate aliquots were withdrawn at intervals and the plasma was assayed for choline (see text for details).
British Journal of Anaesthesia  , DOI: ( /bja/ )
Copyright © 2000 British Journal of Anaesthesia Terms and Conditions