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Progress in sequencing chromosome 6

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Presentation on theme: "Progress in sequencing chromosome 6"— Presentation transcript:

1 Progress in sequencing chromosome 6
- State of the art - Selection of minimal overlapping BAC clones from tomato chr.6 using BAC-end sequences René Klein Lankhorst Plant Research International Wageningen, NL Sept 2005

2 CBSG Tomato Sequencing Program State of the Art for chromosome 6:
Total number of finished BACs: 21 (19 in Phase 1/2 and 2 in Phase 3) Number of BACs in current sequencing pipeline: 14 Number of BACs in current FISH pipeline:11 Sequences of 16 BACs have been uploaded to SGN for public release

3 Selection of BACs by BAC-end screening
Seed BAC BlastN BAC-ends Sequence alignment of selected BAC-ends Pre-selected BACs +0/+0 AFLP fingerprint BAC binning High-Resolution local physical map Largest extending, minimal overlapping BAC

4 Selection of BACs by BAC-end screening
+0/+0 fingerprinting, binning and physical mapping AFLP Finished seed BAC Blast homology search and sequence alignment of BAC ends Sequencing, assembly, walking Selection for minimal overlap and largest extension

5 BlastN analysis of finished seed BAC P250I21
Selection of BACs (I) BlastN analysis of finished seed BAC P250I21 Mi-2 hsp’s Hyp. protein transposon Candidate BAC

6 Selection of BACs (III) P073H07 assembled BAC end on P250I21

7 Assembly of BAC ends on P250I21
Selection of BACs (IV) Assembly of BAC ends on P250I21

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