1. Status of the US contribution to the international
Tomato genome sequencing project:
Chromosomes 1 and 10
Jim Giovannoni, BTI/USDA/Cornell
S.Stack
L. Anderson

2. BAC libraries and BAC end sequences
50000
75000
Additional ordered libraries:
S. cheesmannii HindIII pBeloBAC ,000 clones >100kb avg.
S. pennellii HindIII pBeloBAC ,000 clones >100kb avg.
S. lycopersicum Sau3A cosmid 200,000 clones 20 kb avg.
S. lycopersicum Sau3A cosmid >100,000 clones > 20 kb avg.
S. lycopersicum sheared fosmid 300,000 clones 40 kb avg.
OVERGO anchored markers - apx. 800
BAC-FISH hybridization/localization > 170

3. S.Stack
L. Anderson

4. Tomato BACs FISHed to Chromosomes 1 an 10
UPDATE
CH 1
CH 10

6. 71%
42%
48%

7. Pending Proposal to National Science Foundation
Lukas Mueller, Boyce Thompson Institute
Steve Stack, Colorado State University
Joyce VanEck, Boyce Thompson Institute
Steve Tanksley, Cornell University
Bruce Roe, University of Oklahoma
Jim Giovannoni, BTI/USDA
Continued support and expansion of SGN
FISH localizations
end sequencing of remaining 200,000 fosmid clones
Phase 3 sequencing of 600 BACs
Education and outreach
454 primer-tagged sequencing

8. Position of chromosome 1 and 10 sequencing project
23 BACs completed to date
24 BACs entered 454 pipeline this week
53 additional anchored BACs verified and ready to sequence
16 BAC end extension BACs
~30 anchor BAC extensions based on physical map
30 additional anchored BACs in verification
S.Stack
L. Anderson

9. PLEASE deposit any available BAC sequences by the end of the month!
Special thanks to Joyce VanEck
S.Stack
L. Anderson