1. FLT Given a photosynthesis role play
I will be able to model the steps of the light reaction and calvin cycle
through manipulates and partner

4. Light Reaction

5. Everyone will take part in our photosynthesis theather Part 1--Actors 1) Photosystem II 2) ETC 3) Last ETC 4) Photosystem I 5) ATP synthase 6) Sun 7) Stroma 8) Water 9) ADP (also in part III) 10) NADP+(also in part III) 11) Thylakoid space

6. Roles for Part I
Sun: Holds flashlight and shines it on Photosystems PS II: passes e- cards to last ETC ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space PS I: Passes e- cards to LAST ETC WATER: Passes e- cards to PS II THYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthase ATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP. LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+
ACT OUT ETC first. Where is this happening? What are the jobs? Where do electrons come from to replace those lost from PS II? Where do ATP and NADPH go next?

7. Arrange yourselves
Students wear appropriate name tags and line up in correct position.
Photosystem II, ETC, ETC, ETC, Photosystem I, ETC, ATP synthase stand in a line Sun stands to side of PS II. Stroma stands in front of line. Water stands next to thylakoid space. ADP stands near ATP synthase NADP+ stands near ATP synthase and last ETC

8. Light Reaction

9. Actors—Calvin Cycle 5 Actors: 1 CO2
ATP and NADPH from light reactions 1 Stroma
1 RuBP
Stroma, “Hi, I’m stroma. The Calvin Cycle happens around me”
CO2 enters and high fives stroma, “Hi, I’m CO2” (circle stroma)
RuBP enters and high fives stroma “Hi, I’m RuBP” (circle stroma
CO2 and RuBP link arms . Both say “We are joined by RUBISCO. We are now a 6 carbon molecule. Too bad we are unstable. Let’s become two 3 carbon molecules. Just call us PGA.”
ATP removes its P and says “I am now ADP and are low energy. Back to the light reaction.”
NADPH removes its H+ and passes it and 2 e- cards to STROMA.  “I am now NADP+ and are missing electrons. Back to the light reaction.”
STROMA takes 2 e- and H+ from NADPH, and energy and P from ATP.
STROMA yells ”I need 2 more CO2 and RuBP so I can make G3P and recycle RuBP”

10. Part II
SPLITTING WATER: Assign 8 students to be water molecules in THYLAKOID SPACE. Each starts wearing an O card with 2 H's attached and holds 2 e- cards.
As water is split, the H+ cards are detached and are passed to the THYLAKOID SPACE. The 2 e- cards pass to PS II, and the leftover O holds hands and joins with another O to become O2 and leaves the chloroplast (room).

12. Calvin Cycle

13. Calvin cycle 1C 5C 6C 5C 3C 3C 3C CO2 RuBP RuBisCo 3 ATP 3 ADP used
1. Carbon fixation C
3. Regeneration of RuBP C 5C C
RuBP
RuBisCo C
ribulose bisphosphate 6C
starch, sucrose, cellulose & more
3 ADP
3 ATP C
ribulose bisphosphate carboxylase 5C C
used
to make glucose C
glyceraldehyde-3-P C 3C
PGA
1. A five-carbon sugar molecule called ribulose bisphosphate, or RuBP, is the acceptor that binds CO2 dissolved in the stroma. This process, called CO2 fixation, is catalyzed by the enzyme RuBP carboxylase, forming an unstable six-carbon molecule. This molecule quickly breaks down to give two molecules of the three-carbon 3-phosphoglycerate (3PG), also called phosphoglyceric acid (PGA).
2. The two 3PG molecules are converted into glyceraldehyde 3-phosphate (G3P, a.k.a. phosphoglyceraldehyde, PGAL) molecules, a three-carbon sugar phosphate, by adding a high-energy phosphate group from ATP, then breaking the phosphate bond and adding hydrogen from NADH + H+.
3. Three turns of the cycle, using three molecules of CO2, produces six molecules of G3P. However, only one of the six molecules exits the cycle as an output, while the remaining five enter a complex process that regenerates more RuBP to continue the cycle. Two molecules of G3P, produced by a total of six turns of the cycle, combine to form one molecule of glucose.
G3P C
phosphoglycerate C C C 3C C = C C C
6 ADP
6 ATP
2. Reduction C | H C – C
6 NADP
6 NADPH 3C C 13

14. Calvin cycle when CO2 is abundant
RuBP
ADP
ATP
RuBisCo 6C
unstable
intermediate
G3P
to make
glucose
G3P 5C
C3 plants 3C
PGA 3C
NADP
NADPH
ADP
ATP 3C 14

15. Sun: Holds flashlight and shines it on Photosystems

16. PS II: passes e- cards to last ETC

17. ETC: Passes e- cards along line and pumps H+ cards over shoulder to thylakoid space

18. PS I: Passes e- cards to LAST ETC

19. WATER: Passes e- cards to PS II

20. THYLAKOID SPACE:  Receives H+ cards from ETC and passes H+ cards to ATP synthase

21. ATP SYNTHASE:  receives H+ cards from thylakoid space  and passes them to stroma; holds P cards and attaches them to ADP.

22. LAST ETC: collects all e- cards as they come down line, receives H+ from stroma and gives 2 e- cards and attaches an H+ to NADP+

23. e-

24. ATP SYNTHASE

25. ADENOSINE P P

26. ADENOSINE P P

27. ADENOSINE P P

28. ADENOSINE P P

29. ADENOSINE P P

30. P P P P P P

31. ETC

32. ETC

33. ETC

34. ETC

35. CO2
CO2
CO2
CO2
CO2
CO2

36. CO2
CO2
CO2
CO2
CO2
CO2

37. CO2
CO2
CO2
CO2
CO2
CO2

38. WATER (H2O)

39. NADP +

40. NADP +

41. NADP +

42. NADP +

43. NADP +

44. H+ H+ H+ H+ H+ H+

45. H+ H+ H+ H+ H+ H+

46. H+ H+ H+ H+ H+ H+

47. O

48. PHOTOSYSTEM I

49. PHOTOSYSTEM II

50. CALVIN CYCLE

51. SUN

52. GLUCOSE C C C C C C

53. STROMA

54. THYLAKOID SPACE

55. Rubisco

56. P-C-C-C-C-C-P RuBP

57. P-C-C-C-C-C-P RuBP

58. P-C-C-C-C-C-P RuBP

59. C-C-C-P

60. C-C-C-P

61. C-C-C-P

62. C-C-C-P

63. C-C-C-P

64. C-C-C-P

65. Narrated Photosynthesis

66. Cellular respiration and photosynthesis quiz

67. Make a comic strip explaining photosynthesis
Characters
Chloroplast (stroma + thylakoid)
ATP
Stroma
CO2
Sunlight
H2)
NADP+
NADPH
Membrane Proteins H+
ATP synthase
Glucose
RuBP
Rubisco
Electrons O2