1. Evidence for Circulating Bone Marrow-Derived Endothelial Cells
by Qun Shi, Shahin Rafii, Moses Hong-De Wu, Errol S. Wijelath, Cong Yu, Atsushi Ishida, Yuji Fujita, Sudesh Kothari, Robert Mohle, Lester R. Sauvage, Malcom A.S. Moore, Rainer F. Storb, and William P. Hammond
Blood
Volume 92(2):
July 15, 1998
©1998 by American Society of Hematology

2. Differentiation of CD34+ hematopoietic cells to endothelial cells.
Differentiation of CD34+ hematopoietic cells to endothelial cells. (a) Adherent endothelial colonies formed after 15 to 20 days in culture incubation with VEGF/IGF/bFGF (original magnification × 400). (b) Formation of endothelial monolayer after continuous incubation with VEGF (original magnification × 600). (c) Endothelial monolayers incorporating acetylated LDL (original magnification × 600). (d) CD34+ differentiated cells that stained positively for vWF antigen (original magnification × 600). (e) CD34-selected cells expressing Flk-1 mRNA. BMEC, bone marrow endothelial cells; BM, bone marrow; CB, cord blood; PB, peripheral blood; FL, fetal liver; HUVEC, human umbilical vein endothelial cells.
Qun Shi et al. Blood 1998;92:
©1998 by American Society of Hematology

3. Detection of bone-marrow-derived endothelial cells on vascular prostheses.
Detection of bone-marrow-derived endothelial cells on vascular prostheses. (a) Sensitivity of the microsatellite assay. (b and c) Double labeling with Hoechst and FITC anti-vWF. (d) PCR analysis for (CA)n repeat polymorphism of DNA extracted from (b).
Qun Shi et al. Blood 1998;92:
©1998 by American Society of Hematology

4. PCR genotyping for determination of origin of silver-nitrate-stained endothelial cells.
PCR genotyping for determination of origin of silver-nitrate-stained endothelial cells. (a) Polygonal endothelial cells identified on Dacron grafts after silver nitrate staining. (b) PCR genotyping of silver nitrate-stained endothelial cells demonstrating bone marrow origin. (c) Endothelial cells stained positive for CD34 antigen. (d) Hematoxylin and eosin staining of silver nitrate-stained section.
Qun Shi et al. Blood 1998;92:
©1998 by American Society of Hematology