1. Development and Evaluation of a Pan-Sarcoma Fusion Gene Detection Assay Using the NanoString nCounter Platform 
Kenneth T.E. Chang, Angela Goytain, Tracy Tucker, Aly Karsan, Cheng-Han Lee, Torsten O. Nielsen, Tony L. Ng 
The Journal of Molecular Diagnostics 
Volume 20, Issue 1, Pages (January 2018)
DOI: /j.jmoldx
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Schematic of the probe design for fusion gene detection using the NanoString nCounter Elements platform. In the CodeSet, each fusion junction is represented by three oligonucleotide probes. Probe A binds the RNA transcript across the fusion junction. Probe B binds directly 3′ of probe A on the RNA transcript. A protector probe binds part of probe A when it is not bound to an RNA transcript for increased specificity. A reporter tag containing a unique optical barcode specific to the fusion break point is attached to each probe A for multiplexed analysis. A biotin tag is attached to each probe B for attachment to a streptavidin-coated cartridge.
The Journal of Molecular Diagnostics  , 63-77DOI: ( /j.jmoldx )
Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions