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Adaptamers Thrombin-binding domain Streptavidin-binding domain

Published byKatariina Salonen Modified over 5 years ago

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Presentation on theme: "Adaptamers Thrombin-binding domain Streptavidin-binding domain"— Presentation transcript:

1 Adaptamers Thrombin-binding domain Streptavidin-binding domain
Linking region Thrombin Streptavidin

2 Why develop adaptamers?
To offer a new way to target substrates to cells. To create a tool for studying cell-cell interactions. To provide a foundation for engineered catalysts. happy colorful things.

3 Adaptamer testing Bead surface Adaptamer = A15 Control: no thrombin
streptavidin adaptamer Adaptamer = A15 thrombin anti-thrombin antibody Control: no thrombin Bead surface

4 Adaptamer quenching… + + Results: Displacement strand
change color of displacement strand, stop blinding pp (yellow) No displacement strand added Displacement strand added Background fluorescence +

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