1. Chapter 12. Basic Two-Dimensional Experiments
Why multi-dimensional?
Resolution
Multi-quantum coherences
Coherence transfer
Types of multi-dimensional spectra:
To Correlate
To Resolve
To Exchange (Diffusion)

2. From 1D to 2D, 3D… t2 t1

3. F2
A 2D Spectrum t2
FT2
FT1 F1 t1

8. Acquisition and data processing:F2 F1

16. Invisible terms in the 2nd dimension

23. Recollecting Chapter 2

24. Four basic lineshapes Anti-phase absorptive FT Real part
In-phase dispersive
FT Real part
Anti-phase dispersive
FT Real part
FT Real part
In-phase absorptive

26. A 2D dispersive peak

27. A 2D absorptive peak

29. + -
+ -

32. Absolute Value Display

33. The major drawback of COSY: cross peaks
near the diagonal line may be obscured by
diagonal peaks.

35. TOCSY region of amide protons from residues Lysine 29 and 48

55. DQF COSY

66. Phase Cycling & Selection of Coherence Pathway

69. Effect of phase shift on the density matrix