1. Dendritic cells in semen of infertile men: association with sperm quality and inflammatory status of the epididymis 
Yong-Gang Duan, M.D., Qiongli Zhang, M.D., Yuchen Liu, M.D., Lisha Mou, Ph.D., Gang Li, Ph.D., Yaoting Gui, M.D., Zhiming Cai, M.D., Ph.D. 
Fertility and Sterility 
Volume 101, Issue 1, Pages e3 (January 2014)
DOI: /j.fertnstert
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
CD11c+ HLA-DR+ dendritic cells (DCs) in semen from patients with chronic inflammation of the genital tract (CIGT). (A–D) Dot plots show CD11c+ HLA-DR+ cells detected by flow cytometric analysis. (A and B) For each sample, HLA-DR–Cy5 (x-axis) was used to set a gate to show (A) the isotype control PE-Cy5 and (B) the HLA-DR+ cells. (C and D) Representative dot plots revealing (C) isotype control and (D) CD11c+ cells within the HLA-DR–positive fraction. (E) CD11c+ DCs made up 47.2% (23.68%–63.68%) of HLA-DR+ cells, and a significant difference of CD11c+HLA-DR+ DCs was found in the inflammatory (IN) group compared with the noninflammatory (Non-IN) and fertile groups (P<.01). (F) The number of HLA-DR+ cells showed a significant increase [11.3% (6.19%–19.53%)] in the inflammatory group (P<.01). Data are expressed as median (interquartile range). *P<.01.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Flow cytometric immunophenotyping and cytokines of seminal dendritic cells (DCs) from patients with chronic inflammation of the genital tract (CIGT). Seminal DCs were investigated for the costimulatory molecules (A) CD80, (B) CD86, and (C) CD83 as well as (D) CCR6, (E) CCR7, and (F) CD14, and further detected for intracellular staining of cytokines (G) interleukin (IL) 23p19, (H) tumor necrosis factor [TNF]–related apoptosis-inducing ligand (TRAIL), and (I) TNF-α. Mean value in percentage of experiments ± SEM is given in the right corner (n = 5; red line, the respective subject; compared with isotype control, black line).
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 1
High numbers of dendritic cells (DCs) are present within the semen from patients with chronic inflammation of male genital tract (CIGT). CD11c+ HLA-DR+ DCs were detected in semen from patients with CIGT by double immunofluorescence. (A) CD11c+ cells were stained with Cy2-labeled antibody, and (B) HLA-DR+ cells were detected by Cy3-labeled antibody. (C) Merged: CD11c, green; HLA-DR, red; 4′,6-diamidino-2-phenylindole, blue. Representative pictures from n = 5; ×1,000.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 2
Negative correlation between dendritic cells (DCs) and spermatozoa motility and vitality. A negative correlation was detected between the percentage of CD11c+ HLA-DR+ DCs and sperm (A) motility and (B) vitality in the inflammatory group (n = 32). The mean fluorescence intensity (MFI) of HLA-DR expression, which reflects DC maturity, also is negatively correlated with sperm (C) motility and (D) vitality.
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 3
Correlation between dendritic cells (DCs) and sperm DNA fragmentation index (DFI). The percentage of abnormal spermatozoa with detectable DFI (%) was performed with flow cytometric analysis. (A) The pseudocolor image represents density plots of spermatozoa. (B) The DFI was calculated from the DFI frequency obtained from the ratio between the red and total (red plus green) fluorescence (x-axis, red fluorescence, fragmented DNA; y-axis, green fluorescence, native DNA). A significant correlation between sperm DFI and the percentage of (C) CD11c+ HLA-DR+ DCs and (D) HLA-DR mean fluorescence intensity (MFI) was found in the inflammatory group (n = 32).
Fertility and Sterility  , e3DOI: ( /j.fertnstert )
Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions