Presentation is loading. Please wait.

Presentation is loading. Please wait.

Volume 50, Issue 2, Pages (August 2006)

Similar presentations


Presentation on theme: "Volume 50, Issue 2, Pages (August 2006)"— Presentation transcript:

1 Volume 50, Issue 2, Pages 290-301 (August 2006)
Gene Expression in Normal Urothelium Depends on Location within the Bladder: A Possible Link to Bladder Carcinogenesis  Sharon C. Doherty, Stephanie R. McKeown, Jesus A. Lopez, Ian K. Walsh, Valerie J. McKelvey-Martin  European Urology  Volume 50, Issue 2, Pages (August 2006) DOI: /j.eururo Copyright © 2006 European Association of Urology Terms and Conditions

2 Fig. 1 (a) The R-I plot depict log2-transformed normalized differential-expression levels (log2(ζi)) as a function of log10(GiUO*Ridome). (b) Scatter plots for Cy3-ureteric orifice and Cy5-hybridisation. Plots show the variation in fluorescent signal intensities between the ureteric orifice and dome. The parallel lines flanking the centre diagonal line highlight changes in expression of 2-, 5-, and 10-fold. European Urology  , DOI: ( /j.eururo ) Copyright © 2006 European Association of Urology Terms and Conditions

3 Fig. 2 A strong correlation between the levels of expression of five transcripts in the ureteric orifice versus the dome discovered by microarray analysis and confirmed by RT-PCR. Confirmation analysis by RT-PCR was carried out on five differentially expressed clones uncovered by microarray analysis. The results of RT-PCR were normalised to the expression of r28S or GAPDH in each sample, and expressed as fold change of the ureteric orifice over the expression of the dome. European Urology  , DOI: ( /j.eururo ) Copyright © 2006 European Association of Urology Terms and Conditions


Download ppt "Volume 50, Issue 2, Pages (August 2006)"

Similar presentations


Ads by Google