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Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus,

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Presentation on theme: "Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus,"— Presentation transcript:

1 Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus, Laimonas Griskevicius  The Journal of Molecular Diagnostics  Volume 11, Issue 1, Pages (January 2009) DOI: /jmoldx Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Evaluation of SNP RQ-PCR sensitivity and accuracy. A: Fluorescence versus cycle plots were constructed from serial dilutions of 100, 10, 1, 0.1, and 0.01% of positive patient DNA in negative donor DNA. The total amount of DNA mix was 100 ng. The lowest concentration of SNP-positive template reliably detected in all samples was 0.1%. B: A standard amplification curve was generated by plotting the cycle number versus the concentration. The correlation coefficient between the calculated and the known concentrations was Amplification efficiency calculated from the slope of the curve was 1.17. The Journal of Molecular Diagnostics  , 66-74DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Comparison of chimerism quantification results obtained with short insertion/deletion polymorphism qPCR6 and SNP-specific qPCR (this report) for six different BMT patients. Six different SNP alleles were used for chimerism evaluation by SNP-specific qPCR: rs variant T, rs variant A, rs variant G, rs variant G, rs variant A, and rs variant C. The Journal of Molecular Diagnostics  , 66-74DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Scatterplots with regression analysis (A) and Bland Altman analysis (B) of SNP-specific qPCR versus short insertion/deletion polymorphism qPCR6 (n = 35). The Journal of Molecular Diagnostics  , 66-74DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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