1. Fertility and Sterility
A heparin-binding membrane protein from goat spermatozoa immobilizes spermatozoa in the presence of complement 
Visesato Mor, M.Sc., Tapasi Das, M.Sc., Koela Ray, M.Sc., Tapati Chatterjee, Ph.D. 
Fertility and Sterility 
Volume 85, Pages (April 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Electrophoretic comparison of epididymal sperm surface protein solubilized by detergent CHAPS at 0.1%, 0.25%, 0.5% (wt/vol) lanes 2, 3,and 4, respectively. Gels stained with Coomassie blue. Each lane contained 100 μg of protein. Lane 1: SDS solubilized membrane protein.
Mor. Membrane protein of goat spermatozoa. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Elution pattern of sperm membrane proteins from heparin affinity column, hybridization of heparin-binding sperm membrane protein (HBSM) with [125I]-heparin, and dot-ELISA. (A) Fractions eluted with gradient NaCl concentration stepwise; peak I, II, and III were eluted with 0.25, 0.5, and 1.0 M NaCl solution, respectively. (B) Peak I and II were concentrated and analyzed by 10% (wt/vol) SDS-PAGE with 20 μg of protein per lane. Lane 2, peak I; lane 3, peak II; and lane 1, molecular weight marker. (C) Purified HBSM protein was transferred to nitrocellulose membrane and hybridized with radiolabeled heparin. (D) Dot-ELISA of HBSM. Five micrograms each of HBSM (dot 2 and 4) and 10 mM α-methyl mannopyranoside eluted fraction from ConA column (dot 1 and 3) were dabbed on nitrocellulose membrane and stained with HBSM antisera (dot 1 and 2) and pre-immunized rabbit sera (dots 3 and 4). Color developed with 4-chloro-1-naphthol.
Mor. Membrane protein of goat spermatozoa. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Localization of heparin-binding sperm membrane protein (HBSM) on spermatozoa. Spermatozoa smeared on glass slides were stained with HBSM antisera (C), with pre-immunized rabbit sera (B), and with goat anti-rabbit IgG-HRP conjugate (A). The arrowhead in C indicated the HBSM located at the anterior region of the spermatozoal head. Color developed with 4 chloro-1-naphthol. Original magnification, ×100.
Mor. Membrane protein of goat spermatozoa. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Sperm immobilization test. Heparin-binding sperm membrane protein antisera in presence of complement (♦) or antisera alone (●) SMA2 antibody in presence of complement (▵) and sperm cells in RPMI-1640 medium (□). Each point represents the mean from an experiment repeated five times. Bar (I) symbol represents SD.
Mor. Membrane protein of goat spermatozoa. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions