1. Volume 58, Issue 4, Pages 1492-1499 (October 2000)
Up-regulation of monocyte chemoattractant protein-1 in tubulointerstitial lesions of human diabetic nephropathy 
Takashi Wada, Kengo Furuichi, Norihiko Sakai, Yasunori Iwata, Keiichi Yoshimoto, Miho Shimizu, Shin-Ichi Takeda, Kazuya Takasawa, Mitsuhiro Yoshimura, Hiroshi Kida, Ken-Ichi Kobayashi, Naofumi Mukaida, Takero Naito, Kouji Matsushima, Hitoshi Yokoyama 
Kidney International 
Volume 58, Issue 4, Pages (October 2000)
DOI: /j x
Copyright © 2000 International Society of Nephrology Terms and Conditions

2. Figure 1
Urinary monocyte chemoattractant protein-1 (MCP-1) levels in patients with diabetic nephropathy were significantly elevated. Values are mean ± SEM.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 2
Urinary levels of MCP-1 increased in patients with massive proteinuria. Values are mean ± SEM. **P < 0.01.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 3
Correlation of urinary MCP-1 levels and tubulointerstitial lesions in diabetic nephropathy. Urinary levels of MCP-1 increased in accordance with the damage of interstitial fibrosis (A) or tubular atrophy (B). In addition, there was a significant correlation between urinary MCP-1 levels and the severity of nephrosclerosis (C). Values are mean ± SEM. *P < 0.05; **P < 0.01 by analysis of variance test.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

5. Figure 4
Correlation of urinary MCP-1 levels and glomerular lesions in diabetic nephropathy. Urinary levels of MCP-1 increased in accordance with the damage of glomerular diffuse lesions (A). In addition, the patients with nodular lesions (B), exudative lesions (C), and the presence of mesangiolysis (D) showed significantly higher levels of urinary MCP-1. Values are mean ± SEM. *P < 0.05; **P < 0.01 by analysis of variance test.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

6. Figure 5
Detection of MCP-1 in diabetic nephropathy. Expression of MCP-1 protein in renal tissues was detected using a specific monoclonal anti-human MCP-1 antibody as described in the Methods section. The sections were observed under a light microscope. The representative case who had advanced diabetic nephropathy [diffuse lesion III, nodular lesion (+), exudative lesion (-), mesangiolysis (-), interstitial fibrosis III, tubular atrophy III, nephrosclerosis II] with nephrotic syndrome (4.2 g/day) was shown here. MCP-1–positive cells were observed mainly in the endothelial, tubular epithelial cells (arrowheads), and infiltrated cells in the interstitium (a, ×200). In situ hybridization detected MCP-1 mRNA mainly in cortical tubuli, peritubular capillary endothelial cells, and infiltrated mononuclear cells (arrows) in the interstitium, but it was not detected in the glomeruli (b and c, ×200; n indicates nodular lesion).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions