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Agarose Electrophoresis of Plasmid DNA

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Presentation on theme: "Agarose Electrophoresis of Plasmid DNA"— Presentation transcript:

1 Agarose Electrophoresis of Plasmid DNA

2 Electrophoretic Behavior of Relaxed and Supercoiled Plasmid DNA

3 Electron Micrographs of Circular Duplex DNAs

4 Topological States of Circular DNA

5 Interrelationship between twist and writhe

6 Example of a Modern Plasmid

7 Recognition and Cleavage Sites of Type II Restriction Enzymes

8 Restriction Digest and Restriction Map of Plasmid DNA

9 Electron Micrograph of a Human Metaphase Chromosome

10 Meselson – Stahl Experiment to Establish Mode of Replication

11 Fiber Autoradiography of Replicating DNA

12 Properties of DNA Polymerases I, II, and III of E.coli

13 Domain Structure of DNA Polymerase I

14 Processivity of Various DNA Polymerases

15

16 Space-filling Model of the Crystal structure of the Large Fragment of
E.Coli DNA Polymerase I


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