1. Searching the NCBI Databases

2. Search for CFTR[gene] in the Protein database
What is it?

3. Limit to genes in human only CFTR[gene] AND human[orgn]
How many?

4. Restrict to RefSeq
How many?

5. RefSeq records can also be filtered using the Limits tab

6. Examine the NP_000483 record How many amino-acids are in the protein?
On which chromosome is this protein-coding gene located?

7. Follow the dbSNP link to examine the SNPs associated with this protein
How many SNPs?

8. Follow the dbSNP link to examine the SNPs associated with this protein
Describe mutation in first record. Is it synonymous?

9. Use the Taxonomy Browser to examine fully-sequenced primates genomes

10. Use the Taxonomy Browser to examine fully-sequenced prototheria genomes

11. Sequence Retrieval

12. Search for human BRCA1 in Protein database
Select longest isoform

13. Display the sequences in FASTA format

14. Save the sequence into a WORD file