1. Performance of ACMG-AMP Variant-Interpretation Guidelines among Nine Laboratories in the Clinical Sequencing Exploratory Research Consortium 
Laura M. Amendola, Gail P. Jarvik, Michael C. Leo, Heather M. McLaughlin, Yassmine Akkari, Michelle D. Amaral, Jonathan S. Berg, Sawona Biswas, Kevin M. Bowling, Laura K. Conlin, Greg M. Cooper, Michael O. Dorschner, Matthew C. Dulik, Arezou A. Ghazani, Rajarshi Ghosh, Robert C. Green, Ragan Hart, Carrie Horton, Jennifer J. Johnston, Matthew S. Lebo, Aleksandar Milosavljevic, Jeffrey Ou, Christine M. Pak, Ronak Y. Patel, Sumit Punj, Carolyn Sue Richards, Joseph Salama, Natasha T. Strande, Yaping Yang, Sharon E. Plon, Leslie G. Biesecker, Heidi L. Rehm 
The American Journal of Human Genetics 
Volume 98, Issue 6, Pages (June 2016)
DOI: /j.ajhg
Copyright © 2016 American Society of Human Genetics Terms and Conditions

2. Figure 1
Distribution of Variant-Classification Comparisons according to the Extent of Differences across a Five-Tiered Classification Scheme
(A) Intra-laboratory concordance between laboratory and ACMG-AMP classification systems. This graph compares each site’s use of the ACMG-AMP rules to their own laboratory classification methods.
(B) Inter-laboratory concordance of 97 variants. This graph compares the same calls, based on either the ACMG-AMP rules or the site’s rules, between laboratories.
(C) Inter-laboratory concordance after consensus efforts. This graph shows a final comparison of calls between sites after consensus-building efforts.
The American Journal of Human Genetics  , DOI: ( /j.ajhg )
Copyright © 2016 American Society of Human Genetics Terms and Conditions

3. Figure 2 Distribution of 99 Variants Submitted for Assessment
Gray outlines illustrate the distribution of variant classifications submitted for assessment. Green bars indicate those calls that were agreed upon after initial review, blue bars indicate those calls agreed upon after exchange, and black bars indicate those calls agreed upon after discussion on conference calls.
The American Journal of Human Genetics  , DOI: ( /j.ajhg )
Copyright © 2016 American Society of Human Genetics Terms and Conditions

4. Figure 3 Frequency of Use for Each ACMG Line of Evidence
The American Journal of Human Genetics  , DOI: ( /j.ajhg )
Copyright © 2016 American Society of Human Genetics Terms and Conditions