1. Volume 118, Issue 1, Pages 81-89 (January 2000)
Pyrrolidinedithiocarbamate increases the therapeutic index of 5-fluorouracil in a mouse model 
Simon P. Bach, Rebecca Chinery, Sarah T. O'Dwyer, Christopher S. Potten, Robert J. Coffey, Alastair J.M. Watson 
Gastroenterology 
Volume 118, Issue 1, Pages (January 2000)
DOI: /S (00)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(A, C, E, and G) Apoptotic cell index percentages and (B, D, F, and H) mitotic cell index percentages in large (A-D) and small intestinal crypts (E-H) of BDF 1 mice 6 hours (A, B, E, and F) or 24 hours (C, D, G, and H) after 1 dose of 40 mg/kg 5-FU alone (solid lines) or 40 mg/kg 5-FU plus 250 mg/kg PDTC (dashed lines). Control untreated values are shown for comparison (dotted lines; A, B, E, and F). Six animals were used in each data group, counting 50 half-crypts per mouse. Data lines are smoothed over 3 positions.
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

3. Fig. 1
(A, C, E, and G) Apoptotic cell index percentages and (B, D, F, and H) mitotic cell index percentages in large (A-D) and small intestinal crypts (E-H) of BDF 1 mice 6 hours (A, B, E, and F) or 24 hours (C, D, G, and H) after 1 dose of 40 mg/kg 5-FU alone (solid lines) or 40 mg/kg 5-FU plus 250 mg/kg PDTC (dashed lines). Control untreated values are shown for comparison (dotted lines; A, B, E, and F). Six animals were used in each data group, counting 50 half-crypts per mouse. Data lines are smoothed over 3 positions.
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

4. Fig. 1
(A, C, E, and G) Apoptotic cell index percentages and (B, D, F, and H) mitotic cell index percentages in large (A-D) and small intestinal crypts (E-H) of BDF 1 mice 6 hours (A, B, E, and F) or 24 hours (C, D, G, and H) after 1 dose of 40 mg/kg 5-FU alone (solid lines) or 40 mg/kg 5-FU plus 250 mg/kg PDTC (dashed lines). Control untreated values are shown for comparison (dotted lines; A, B, E, and F). Six animals were used in each data group, counting 50 half-crypts per mouse. Data lines are smoothed over 3 positions.
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

5. Fig. 1
(A, C, E, and G) Apoptotic cell index percentages and (B, D, F, and H) mitotic cell index percentages in large (A-D) and small intestinal crypts (E-H) of BDF 1 mice 6 hours (A, B, E, and F) or 24 hours (C, D, G, and H) after 1 dose of 40 mg/kg 5-FU alone (solid lines) or 40 mg/kg 5-FU plus 250 mg/kg PDTC (dashed lines). Control untreated values are shown for comparison (dotted lines; A, B, E, and F). Six animals were used in each data group, counting 50 half-crypts per mouse. Data lines are smoothed over 3 positions.
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

6. Fig. 2
Time course of small intestinal (A) apoptotic cell index percentage and (B) mitotic cell index percentage after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Data combined from cell positions Arrow indicates a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

7. Fig. 2
Time course of small intestinal (A) apoptotic cell index percentage and (B) mitotic cell index percentage after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Data combined from cell positions Arrow indicates a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

8. Fig. 3
Time course for large intestinal apoptotic cell index percentage in (A) the stem cell zone (cell positions 1-3), (B) the late transit cell zone (cell positions 10-12), and (C) cell positions 1-18, inclusive, after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Arrows indicate a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

9. Fig. 3
Time course for large intestinal apoptotic cell index percentage in (A) the stem cell zone (cell positions 1-3), (B) the late transit cell zone (cell positions 10-12), and (C) cell positions 1-18, inclusive, after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Arrows indicate a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

10. Fig. 3
Time course for large intestinal apoptotic cell index percentage in (A) the stem cell zone (cell positions 1-3), (B) the late transit cell zone (cell positions 10-12), and (C) cell positions 1-18, inclusive, after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Arrows indicate a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

11. Fig. 4
Time course for large intestinal mitotic cell index percentage in (A) the stem cell zone (cell positions 1-3), (B) the late transit cell zone (cell positions 10-12), and (C) cell positions 1-18, inclusive, after a dose of 40 mg/kg 5-FU alone or 40 mg/kg 5-FU plus 250 mg/kg PDTC. Six animals were used at each time point, counting 50 half crypts per mouse. Arrow indicates a significant difference between treatment groups (P < 0.001).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

12. Fig. 5
(A) Colonic epithelium 6 days after administration of 5-FU, 500 mg/kg, in 2 doses 6 hours apart. Arrow indicates a single regenerating crypt containing several mitotic figures (original magnification 400×). (B) Normal murine colonic epithelium (400×). (C) Apoptotic body (thick arrow) and mitotic figure (thin arrow) in colonic epithelium 24 hours after administration of 40 mg/kg 5-FU (1000×). (D) Apoptotic body stained using the TUNEL technique (thick arrow). Note the apoptotic body, which remains unstained (thin arrow; 1000×).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions

13. Fig. 6
(A) Crypt survival curves obtained from the large intestine of BDF 1 mice 6 days after treatment with 5-FU ( mg/kg) alone or 5-FU ( mg/kg) plus PDTC (500 mg/kg), administered as 2 divided doses 6 hours apart. The fraction of surviving colonic crypts is higher when 5-FU was administered with PDTC (P < 0.001, F test). (B) Corresponding data from the small intestine of BDF 1 mice viewed 5 days after treatment described in A. The fraction of surviving crypts is not altered by pretreatment with PDTC (P = 0.62, F test).
Gastroenterology  , 81-89DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions