Aquaphotomics Water Spectral Pattern as　 Holistic Bio Marker and a Collective Mirror Roumiana Tsenkova Kobe University, Japan rtsen@kobe-u.ac.jp.

Aquaphotomics Water Spectral Pattern as　 Holistic Bio Marker and a Collective Mirror
Roumiana Tsenkova Kobe University, Japan

水　と光

Paracelsus in the 16th century said that “water was the matrix of the world and of all its creatures.” The properties of liquid water that governs processes such as aqueous solvation and the transport of protons arise from the motions of water molecules within a constantly changing network of hydrogen bonds.

water and electromagnetic field
Albert Szent-Gyorgyi Biology did not succeed in understanding basic living functions because it focused its attention to a substance in the form of particles dissecting them from two matrixes: water and electromagnetic field Szent-Gyorgyi, A. Bioenergetics. Academic Press, New York. 1957

“Life is water dancing to the tune of solids” Albert Szent-Georgyi and …. the energy fields (RTs)

LIGHT “turns on” the mirror, LIGHT “turns on”
the water in to 3 - dimensional molecular mirror LIGHT as a source of perturbation (electromagnetic field ) and a probe (the spectrum)

Light Absorbed by Water
NIR range overtone region IR range fundamental frequencies

Sun Light Intensity Kita Kyushu 2014 JAACT

NIRS Bench Type Instrument

Portable Instruments &
Fiber Probes For Tissue Measurements Bio Monitoring & Bio Diagnosis

Absorbance Wavelengths, nm

Water Dynamic Spectra 2D And 3D Synchronous Correlation Maps
represent positive correlation represent negative correlation

Water Dynamic Spectra Power Spectra At 1530nm

LIGHT “turns on” the mirror, LIGHT “turns on” the water in to a
molecular mirror All the electromagnetic spectrum is LIGHT

Aquaphotomics Concept Water as Molecular Mirror

What is Aquaphotomics?

Aquaphotomics Concept
Current methodology: reductionism Aquaphotomics: Complex systems monitoring single parameters monitoring water molecular system

AQUAPHOTOMICS Aqua : water Photo : light Omics : all about,
complement of something

The – omics disciplines describe the players, the single molecules in a system. Aquaphotomics describes the environment, the stage, the inter connections.

The rest of – omics disciplines and Aquaphotomics are complimentary, like the Yin and Yang

How does it work?

Activated Water Absorbance Bands
AQUAPHOTOMICS: Water as a Molecular　Mirror Spectral Data Base Bio・Aqueous System NIR Spectrum WAMACS Activated Water Absorbance Bands water Perturbations Water Absorbance Spectral Pattern, WASP WAMACS = Water Matrix Coordinates, i.e. water absorbance bands in VIS-NIR range

the entire complements of water matrix coordinates
AQUAPHOTOME is the entire complements of water matrix coordinates found under various perturbations

Water Matrix Coordinates:
WAMACS Water Matrix Coordinates: Water absorbance bands corresponding to pools of water molecules with the same vibrational frequency

C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 1336～1348 1360～1366 1380～1388 1421～1430 1448～1454 1472～1482 1506～1516 1370～1376 1398～1418 1432～1444 1462～1468 1482～1495

Assignment C (1342)nm – ν3 1344nm H2O - 2* ν3 (Siesler, Ozaki, Kawata, & Heise, 2001) 1340nm liquid water/moisture (Williams, 2009) C (1364)nm – OH-(H2O)1, OH-(H2O)2, OH-(H2O)4 1360nm 1st overtone free OH stretch (OH-(H2O)4) cm-1 (Robertson, Diken, Price, Shin, & Johnson, 2003) 1366nm 1st overtone OH- stretch (OH-(H2O)2) cm-1 (Robertson et al., 2003) C (1374)nm – ν1 + ν3 1379.3nm H2O - ν1 + ν3 – 7250cm-1 (Siesler et al., 2001) 1379.3nm overtone of stretching vibration – 7250cm-1 (Kuroda, Hamano, Mori, Yoshikawa, & Nagao, 2000) 1373 nm first overtone of 2νOH (Lakshmi Reddy, Padma Suvarna, Udayabhaska Reddy, Endo, & Frost, 2014) C (1384)nm – OH-(H2O)1, OH-(H2O)4, O2-(H2O)4 nm 1st overtone interwater / DD stretch (OH-(H2O)4) cm-1 (Robertson et al., 2003) 1381nm H2O-ν1+ ν3 (Cattaneo, Cabassi, Profaizer, & Giangiacomo, 2009)

Water Absorbance Pattern, WAP AQUAGRAM
Aquagram was devised to visualize the WASP. The aquagram displays normalized absorbance values at specific water bands on the axes originating from the center of the graph. Absorbance values at the WAMACs are placed on the respective radial axes.

WATER and VAPOR SPECTRA and AQUGRAM
MPA VAPOR WATER A : Absorbance after EMSC ( nm) μ：Mean of Averaged spectra σ：SD of absorbance each wave length

Self – similarity in Water:
Spectroscopic study In memory of Emilio Del Giudice

Water Spectral Changes at Various Temperatures and Nafion in Water
Self Similarity Study Water Spectral Changes at Various Temperatures (with Zoltan Kovac and Giuseppe Vitiello) and Nafion in Water (with Zoltan Kovac, Antoanella De Ninno and Giuseppe Vitiello)

When energy is absorbed from the vacuum field, the particles will begin to oscillate between two configurations. Liquid water is a two-fluid system [5] (in analogy with superfluid helium) consisting of a coherent phase (about 40 percent of total volume at room temperature) and an incoherent phase

Need of the linear detrend transformation
Fitted linear models (with least squares) raw spectra corrected spectra Various perturbations can cause different slope and/or offset of the baseline of the spectra which can hide the small differences occur in the water spectral pattern spectra are rotated and shifted to set the slop and offset of the fitted linear models to 0

LogTemp vs OD with detrend for DiW first consecutive

Nafion LogEC vs OD with detrend first consecutive
Nafion Log EC vs OD Detrend first consecutive Nafion LogEC vs OD with detrend first consecutive

R for DiW first consecutive

R for MQ first consecutive

R for Nafion Log EC vs OD, First Consecutive

logEC ~ wavelengths 5th consecutive

R for Nafion pH vs OD, First Consecutive

pH ~ wavelengths 5th consecutive

A collaborative experimental and theoretical study on the dependence of the infrared (IR) spectrum of hydrated Nafion electrolyte membrane on the hydration number is investigated in great detail. Experimental time-resolved attenuated total reflection Fourier transform IR spectroscopic results show that Nafion membrane has a unique IR peak intensity dependence on the hydration number. Calculated IR spectra indicate that this unique IR peak intensity dependence is correctly reproduced not for the singly hydrated Nafion but for the doubly hydrated Nafion. This result strongly supports the relay mechanism of the proton conductance, in which protonated water clusters are relayed by the side chains through the doubly hydrated sulfonic acid groups under low-humidity conditions. Experimental and Theoretical Infrared Spectroscopic Study on Hydrated Nafion Membrane Raman K. Singh, Keiji Kunimatsu, Kenji Miyatake, and Takao Tsuneda* Fuel Cell Nanomaterials Centre, University of Yamanashi, Kofu , Japan Macromolecules, 2016, 49 (17), pp 6621–6629

Water Coherence WATER WATER + NAFION 1371 1442 1557 1372.5 1439 1555
Assignment 1364nm TP, OH-(H2O) 1441 – TP, H5O2 H5O2+ (Zundel cation) TP , H9O4+ (Eigen cation) 1806 publication “Theory of decomposition of liquids by electrical currents”, Theodor Grotthuss <100nm <100nm 1443.5 1364.5 1550 1443.5 1550 1452 1545 1362 1452 1547 1362.5 1367.5 1447 1554 1354.5 1441.5 1547 1355 1442 1548 WATER + NAFION 1447.5 1553 1366.5 ～100nm ～100nm

MQ Water, Peaks Found in R for Temperature
WC0 WC1 WC2 WC3 WC4 WC5 WC6 WC7 WC8 WC9 WC10 WC11 WC12 WC13 | | | | 1stCons r 1.00 | | | | 2ndCons 0.50 | | | | 3rdCons 0.00 | | | | 4thCons -0.50 | | | | -1.00 5thCons 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 | TP

DiW Water, Peaks Found in R for Temperature
WC0 WC1 WC2 WC3 WC4 WC5 WC6 WC7 WC8 WC9 WC10 WC11 WC12 WC13 | | | | | | | 1stCons r 1.00 | | | | | 2ndCons 0.50 | | | | | 3rdCons 0.00 | | | | | 4thCons -0.50 | | | | -1.00 5thCons | TP 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600

| | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
Nafion, Peaks Found in R for pH WC0 WC1 WC2 WC3 WC4 WC5 WC6 WC7 WC8 WC9 WC10 WC11 WC12 WC13 | 1stCons r 1.00 2ndCons 0.50 3rdCons 0.00 | | | | | | | | | | | | | | | | | | | | 4thCons -0.50 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | -1.00 5thCons 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 | TP

水の音光の遊び生きている Sound of a stream, Sunlight dancing on waters,
　光の遊び　　　生きている Sound of a stream, Sunlight dancing on waters, Life wakes up again. R. Tsenkova, 2004

Aquaphotomics Understanding Water in Biology
Second International Symposium 26 ~ 29 November, 2016 Kobe University Centennial Hall (Rokko Hall) 1-1 Rokkodai, Nada-ku, Kobe For registration and more information visit:

Aquaphotomics Water Spectral Pattern as　 Holistic Bio Marker and a Collective Mirror Roumiana Tsenkova Kobe University, Japan rtsen@kobe-u.ac.jp.

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Aquaphotomics Water Spectral Pattern as Holistic Bio Marker and a Collective Mirror Roumiana Tsenkova Kobe University, Japan rtsen@kobe-u.ac.jp.

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Aquaphotomics Water Spectral Pattern as　 Holistic Bio Marker and a Collective Mirror Roumiana Tsenkova Kobe University, Japan rtsen@kobe-u.ac.jp.

Presentation on theme: "Aquaphotomics Water Spectral Pattern as　 Holistic Bio Marker and a Collective Mirror Roumiana Tsenkova Kobe University, Japan rtsen@kobe-u.ac.jp."— Presentation transcript: