1. Strategies for annotation of a genome
Approaches
Updating GO annotations

2. Approaches for annotation of a genome
Automated approaches
2. Manual approaches
a. InterPro2GO
a. Sequence similarity
b. Experimental evidence

3. Annotations via sequence similarity
Alignments published in literature
Analysis using full length protein

4. Annotation from experimental evidence
Focus on known genes
Identify literature relevant to that gene
a. using gene names, species name
Complete annotation set for a gene
Annotate all available experimental data
“unknown” annotations ok

5. Updating GO annotations
GO annotations will never be “done”
Ongoing process
New experimental data
More specific annotation
Replace obsolete terms