1. Merkel cell polyomavirus in Merkel cell carcinoma from a Brazilian epidermodysplasia verruciformis patient 
Walmar R. Oliveira, MD, PhD, Mirian N. Sotto, MD, PhD, Cyro Festa, MD, PhD, Peter Rady, PhD, Stephen K. Tyring, MD, PhD 
Journal of the American Academy of Dermatology 
Volume 62, Issue 5, Pages (May 2010)
DOI: /j.jaad
Copyright © 2009 American Academy of Dermatology, Inc. Terms and Conditions

2. Fig 1
Clinical and morphologic aspects of the disease. A, Red-brown, pityriasis versicolor–like lesions on the trunk and arms. B, Merkel cell carcinoma. A pink-red nodule on the trunk. C, Immunolabeling for chromogranin shows dot-like cytoplasmic reactivity within tumor cells.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2009 American Academy of Dermatology, Inc. Terms and Conditions

3. Fig 2
Detection of Merkel cell polyomavirus (MCV) DNA using polymerase chain reaction (PCR) in a Brazilian patient with Merkel cell carcinoma (MCC). The PCR primers were minor modifications of the primer set LT3 (formerly described by Feng et al).3 PCR products were analyzed on 2.0% agarose gel electrophoresis and visualized on an ultraviolet transilluminator. A, Lane M shows the fX174RF DNA marker (Promega Co); lane 1 shows MCC; lane 2 shows MCV-negative control DNA extracted from peripheral blood mononuclear cells (Promega Co); and lane 3 shows the reagent control. The expected 309bp PCR product of MCV can be seen in lane 1. B, Beta-globin reference gene PCR assays. Lane M shows the fX174RF DNA marker; lane 1 shows MCC; lane 2 shows the MCV-negative control DNA extracted from peripheral blood mononuclear cells (Promega Co); and lane 3 shows the reagent control. The expected beta-globin 268bp PCR fragments can be seen in lanes 1 and 2.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2009 American Academy of Dermatology, Inc. Terms and Conditions