1. Drosophila melanogaster

2. 1-2 days
4 days
1-2 days
First instar
1-2 days
1-2 days
Third instar
Second instar
1-2 days

3. pupae Small, oval, two filaments at one end,
laid on the surface of the food
Instar/larvae
Worm-like, eats constantly, burrows in food, black mouth parts, sheds skin twice as it grows.
egg
Larva climbs up side
of tube to pupate,
Outer covering harder and darker, you can
see eyes, legs, wings…the darker they get
the closer they are to hatching.
pupae

4. Adult Emerge from case, fragile, light in color,
the lighter the newest hatched, wings
folded at first, darkens in a few hours,
live about 5-6 weeks.
Females can mate after
emerging from case in
10-12 hours. She can store
sperm in receptacles
every mating for her entire
lifespan, eggs are fertilized
as they are laid.
Adult

5. Male Female Sex combs *concentrated dark banding
with a solid tip on abdomen
*smaller
*Larger
*striped abdomen
*broader abdomen

8. Four chromosomes : 3 Autosomal
and one sex chromosome

10. Mating Behavior

12. A Female laying an egg

13. Wild type (+)

14. Red round eye
Normal long wing
antenae
Tan body hair

15. Vestigal wing
(can’t fly)

16. There are many mutations

17. Caring for Fruit flies

18. Supplies needed
Special tubes
Yeast to feed adults
Petri dish
Paint brushes

19. Paint brush to move flies
Label tubes
Tube with food
-one scoop food
To equal water
(potato and banana
meal)
Sponge cork

20. Sponge cork to keep flies
In and still exchange gasses
Formula blue food:
blue dye to see white
larva easier, larva
eat and tunnel in it
Yeast sprinkled on
food for the adults

21. Anesthetic to put flies to sleep in order to view and record traits.
*the pipe cleaner “wand” in to the flynap and insert in a
sponge to hang in the flynap tube.
*flies to be “napped” need to be transferred to a dry tube without
food before “napping”.
*smells interesting, will not put you to sleep!

22. Putting flies to sleep

23. Putting the “sleeping” flies on a clean dry dish

24. “sleeping” flies in the dish to view

25. Separating flies gently with a paintbrush

26. Viewing flies with a stereoscope

27. You must use a stereoscope to determine sex of the flies

28. Female
Male

29. Be sure to label all tubes

30. Fly trap: jar/beaker with paper funnel and vinegar
attracts escapees.

31. Alcohol rubbing
Fly jar: to place flies no longer needed
*please bring a jar- one per group

33. What will you be doing?
*Testing one variable on gender development
*investigating your topic: background research
*Designing a procedure to test your hypothesis
*determining sex of fruit flies and collecting data,
analyzing data, writing a result and conclusion
*designing a display board of your project

34. Problem Does ____________affect gender
development in Drosophila melanogster?
*Temperature
*Acidity
*Amount of light

35. Hypothesis *after a little research, make an educated guess
to your problem statement.
If _______________, then ____________.

36. Designing an Experiment
Control
Variable
Constants
Sample size
Bias free
6. Data

37. Today *Investigate the flies with a stereoscope.
*try to separate flies into male and female, move them gently
with the paint brush