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Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition  Monica Faut, Evelin Mariel Elia,

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Presentation on theme: "Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition  Monica Faut, Evelin Mariel Elia,"— Presentation transcript:

1 Peroxisome proliferator-activated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition  Monica Faut, Evelin Mariel Elia, Fernanda Parborell, Ph.D., Noelia Melina Cugnata, Marta Tesone, Ph.D., Alicia Beatriz Motta, Ph.D.  Fertility and Sterility  Volume 95, Issue 1, Pages (January 2011) DOI: /j.fertnstert Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 (A) Protein expression of follicular StAR from control, eCG-treated, and eCG+DHEA-treated rats, a representative Western blot, and the graph corresponding to the integrated optical density of the bands. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b, a vs. c, and b vs. c. ∗P < by analysis of variance. (B) MRNA expression of StAR of the same three groups and the graph of integrated optical density bands. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < was significantly different from control value by analysis of variance. (C) Protein expression of follicular PPARγ from control, eCG-treated, and eCG+DHEA-treated rats. A representative Western blot and the corresponding graph is shown. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < was significantly different from control value by analysis of variance. (D) The mRNA expression of PPARγ of the three groups and the corresponding graph. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b and a vs. c, P < 0.001; b vs. c, P < 0.05 by analysis of variance. (E) A representative dot plot and the quantitative estimation of viability and apoptosis. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. c, P < 0.05; b vs. d, P < by analysis of variance. (F) Agarose gel showing DNA fragmentation and the quantitative estimation of DNA cleavage. Data points represent the mean ± SEM of four independent gel runs: a vs. b, P < 0.01. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3 Figure 1 (A) Protein expression of follicular StAR from control, eCG-treated, and eCG+DHEA-treated rats, a representative Western blot, and the graph corresponding to the integrated optical density of the bands. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b, a vs. c, and b vs. c. ∗P < by analysis of variance. (B) MRNA expression of StAR of the same three groups and the graph of integrated optical density bands. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < was significantly different from control value by analysis of variance. (C) Protein expression of follicular PPARγ from control, eCG-treated, and eCG+DHEA-treated rats. A representative Western blot and the corresponding graph is shown. Each column represents the mean ± SEM of 10 measurements from different animals. ∗P < was significantly different from control value by analysis of variance. (D) The mRNA expression of PPARγ of the three groups and the corresponding graph. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. b and a vs. c, P < 0.001; b vs. c, P < 0.05 by analysis of variance. (E) A representative dot plot and the quantitative estimation of viability and apoptosis. Each column represents the mean ± SEM of 10 measurements from different animals: a vs. c, P < 0.05; b vs. d, P < by analysis of variance. (F) Agarose gel showing DNA fragmentation and the quantitative estimation of DNA cleavage. Data points represent the mean ± SEM of four independent gel runs: a vs. b, P < 0.01. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions


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