1. Design a novel 3-layered nanofibrous mat for wound healing
Fereshteh Nejaddehbashi 1*, Mahmoud Orazizadeh 1&2, Mohammadreza Abbaspour3, Mahmoud Hashemitabar1&2, Vahid Bayati1&2, Eskandar moghimipour1&4
1. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences.
2. Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences.
3. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences.
 4. Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences.
Tensile test
The elasticity modulus of the 3-layered nanofibrous mat with 500 µm thickness was 15.7 Mpa, which is within the range of 15–50 Mpa of native human skin [3].
Objectives
Skin, as the largest organ in the body provides a barrier between the body and external environment. In the recent decades, tissue engineering researchers have utilized several methods to create constructs that mimic the extracellular matrix (ECM) of skin. In skin tissue engineering, different synthetic and biological materials have been used to produce fundamental scaffolds for skin repairing [1, 2].
The purpose of this study was to prepare a 3-layered biomimetic nanofibrous mat, composed of PCL/SSD in the bottom, CS/PEO in the middle, and PCL/Coll in the top layer.
Fig.4. Proliferation of cells on mat in the absence of SSD (a1, a2 and a3) and presence of SSD (b1, b2 and b3).
Fig.2. Stress – strain curve of 3-layered nanofibrous mat.
Methods
TGA analysis
TGA curves of 3-layered nanofibrous mat showed the initial decomposition temperature around 300°C.
Field emission electron microscopy (FE-SEM), mechanical properties, thermogravity analysis (TGA) and MTT assay were applied for characterization of the 3-layered nanofibrous mat.
Conclusions
This study demonstrated that blended mat could present as a candidate for more effective repairing of skin tissue in future.
Results
Characterization of nanofibers
The FE-SEM analysis showed that the electrospinning process with optimized parameters led to formation of uniform nanofibers for each layer.
References
Biazar E. Application of polymeric nanofibers in medical designs, part I: Skin and eye. International Journal of Polymeric Materials and Polymeric Biomaterials 2017; 66(10):
Das S, Baker AB. Biomaterials and nanotherapeutics for enhancing skin wound healing. Frontiers in bioengineering and biotechnology 2016; 4.
Ulubayram K, Cakar AN, Korkusuz P, Ertan C, Hasirci N. EGF containing gelatin-based wound dressings. Biomaterials 2001; 22(11):
Fig.3.TGA curves of 3-layered nanofibrous mat.
Cell proliferation
MTT assay showed that nanofibrous mats containing SSD and lacking SSD were capable of cell growth.
Fig. 1.FE-SEM of three layers of scaffold are presented. PCL/SSD (A), PEO/CS (B), and PCL/Coll(C).