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Volume 119, Issue 3, Pages (September 2000)

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1 Volume 119, Issue 3, Pages 624-630 (September 2000)
Persistent genetic abnormalities in Barrett's esophagus after photodynamic therapy  Kausilia K. Krishnadath, Kenneth K. Wang, Ken Taniguchi, Thomas J. Sebo, Navtej S. Buttar, Marlys A. Anderson, Lori S. Lutzke, Wanguo Liu  Gastroenterology  Volume 119, Issue 3, Pages (September 2000) DOI: /gast Copyright © 2000 American Gastroenterological Association Terms and Conditions

2 Fig. 1 (A) H&E-stained Barrett's esophagus biopsy specimen showing high-grade dysplasia at the end of follow-up after PDT. (B) Immunohistochemistry for Ki-67 with the Mib-1 antibody showing increased proliferation in epithelial cells. (C) p53 protein overexpression assessed by immunohistochemistry with the DO-7 antibody shows intense staining for p53 in virtually all nuclei. (Original magnification 10×.) Gastroenterology  , DOI: ( /gast ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

3 Fig. 2 (A) Feulgen-stained Barrett's esophagus biopsy specimen for image analysis. (B) Bar histogram showing diploid and tetraploid peak as measured by Cas 200 image analysis system. Gastroenterology  , DOI: ( /gast ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

4 Fig. 3 (A) DHPLC elution profile of exon 6 of normal gastric DNA showing a homoduplex profile. (B) DHPLC of exon 6 of a Barrett's esophagus biopsy specimen after PDT showing a heteroduplex profile (arrow). Gastroenterology  , DOI: ( /gast ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

5 Fig. 4 Methylation-specific PCR for the p16 promotor of bisulfate-treated DNA showing a methylation-specific product in Barrett's esophagus biopsy specimens taken before and after PDT. The normal control does not show methylation. Gastroenterology  , DOI: ( /gast ) Copyright © 2000 American Gastroenterological Association Terms and Conditions


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