1. a b
Spike IL-17AA pg/mL
Dilution Factor (DF)
Recovery pg/mL
Endogenous pg/mL
DF Corrected pg/mL
% Recovery
% Linearity
Spike IL-17FF pg/mL
CSF1: 6.25 1
6.90
0.05
110
5.26
0.03 84 2
2.82
5.64 90 82
2.55
5.10 97 4
1.51
6.04
107
1.29
5.16 83
101 8
0.81
6.48
104
108
0.69
5.52 88
CSF2: 6.25
7.36
0.25
118
5.60
0.06
3.42
6.84
109 93
2.64
5.28 94
1.75
7.00
112
103
1.34
5.36 86
0.88
7.04
113
100
0.66
CSF3: 6.25
6.97
0.24
5.77 92
3.30
6.60
106 95
2.71
5.42 87
1.71
1.37
5.48
0.87
6.96
111
CSF4: 6.25
6.24
0.08
5.62
3.03
6.06
2.62
5.24
1.56
105
0.72
5.76
CSF5: 6.25
5.68 91
5.59
0.04 89
2.89
5.78
102
2.56
5.12
1.43
5.72 98
1.28
0.86
6.88
120
0.65
5.20
Table S4. Linearity of dilution of recombinant IL-17AA (a) and IL-17FF (b) in CSF.
Spike Recovery is calculated as: (Measured Mean [IL-17FF]-endogenous [IL-17FF] / expected Spike [IL-17FF])*100
Linearity of dilution is calculated as: (Measured Mean [IL-17FF]*2/ concentration of the previous dilution)*100
All samples and dilutions met the acceptability criterion of being within ± 20% of the previous dilution,
and recoveries were within ±20% of nominal.