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Published byCassandra Avice Goodman Modified over 6 years ago
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Both Carboxy-Terminal Tails of α- and β-Tubulin Are Essential, but Either One Will Suffice
Jianming Duan, Martin A. Gorovsky Current Biology Volume 12, Issue 4, Pages (February 2002) DOI: /S (02)
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Figure 1 Glycylation Status of Tubulins from Viable Mutant Strains
Proteins were extracted from the viable strains described in Table 2 and analyzed by Western blotting with R-polygly or a mixture of α- and β-tubulin antibodies (see Experimental Procedures). The rescued mutants showed a marked decrease in the polyglycylation of β-tubulin and significant increase of the polyglycylation in α-tubulin compared with the wild-type strain. Increased levels of α glycylation and the resulting decreased electrophoretic mobility of α isoforms have also been observed in two dimensional gel analyses (J. Gaertig and Y. Gao, personal communication). Current Biology , DOI: ( /S (02) )
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